"
Team:Queens-Canada/10 June 2010
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | {{:Team:Queens-Canada/head}} | |
| - | + | ||
| + | <h2>June 10, 2010</h2> | ||
| + | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Hao and Thai | Hao and Thai | ||
| Line 9: | Line 11: | ||
Mistakes during gel loading are possible. | Mistakes during gel loading are possible. | ||
| - | + | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | |
Hao and Thai | Hao and Thai | ||
'''pIC54''' and '''pIC691''' digestion products | '''pIC54''' and '''pIC691''' digestion products | ||
| - | + | <h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3> | |
Yuli | Yuli | ||
| Line 23: | Line 25: | ||
Products stored in -80 °C freezer. | Products stored in -80 °C freezer. | ||
| - | + | <h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | |
Steve and Thai | Steve and Thai | ||
Revision as of 05:14, 27 October 2010
June 10, 2010
Gel Electrophoresis
Hao and Thai
pSip-1, pUnc-47
Used 0.45g agarose in 65 mL TBE. Results: lanes 1,2,5, and 9 were visible. The rest of the gel was blank. The primers for unc-47 will definitely have to be replaced. Mistakes during gel loading are possible.
Gel Electrophoresis
Hao and Thai
pIC54 and pIC691 digestion products
Glycerol Stock
Yuli
pIC691 and pIC54
Change: 250 µL of 80% glycerol was used with 750 µL of culture.
Products stored in -80 °C freezer.
PCR
Steve and Thai
pIC691, pIC54
Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C. Products stored in -20 °C freezer.
