Team:Queens-Canada/10 June 2010

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(Difference between revisions)
(New page: ==June 10, 2010== ====Gel==== Hao and Thai '''pSip-1''', '''pUnc-47''' Used 0.45g agarose in 65 mL TBE. Results: lanes 1,2,5, and 9 were visible. The rest of the gel was blank. The primer...)
 
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==June 10, 2010==
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{{:Team:Queens-Canada/head}}
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====Gel====
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<h2>June 10, 2010</h2>
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
Hao and Thai
Hao and Thai
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'''pSip-1''', '''pUnc-47'''
'''pSip-1''', '''pUnc-47'''
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Mistakes during gel loading are possible.
Mistakes during gel loading are possible.
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====Gel====
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
Hao and Thai
Hao and Thai
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'''pIC54''' and '''pIC691''' digestion products
'''pIC54''' and '''pIC691''' digestion products
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====Glycerol Stock====
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<h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3>
Yuli
Yuli
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'''pIC691''' and '''pIC54'''
'''pIC691''' and '''pIC54'''
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Products stored in -80 °C freezer.
Products stored in -80 °C freezer.
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====PCR====
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<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3>
Steve and Thai
Steve and Thai
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'''pIC691''', '''pIC54'''
'''pIC691''', '''pIC54'''
Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C.  
Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C.  
Products stored in -20 °C freezer.
Products stored in -20 °C freezer.
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{{:Team:Queens-Canada/foot}}

Latest revision as of 05:19, 27 October 2010

June 10, 2010

Gel Electrophoresis

Hao and Thai

pSip-1, pUnc-47

Used 0.45g agarose in 65 mL TBE. Results: lanes 1,2,5, and 9 were visible. The rest of the gel was blank. The primers for unc-47 will definitely have to be replaced. Mistakes during gel loading are possible.

Gel Electrophoresis

Hao and Thai

pIC54 and pIC691 digestion products

Glycerol Stock

Yuli

pIC691 and pIC54

Change: 250 µL of 80% glycerol was used with 750 µL of culture.

Products stored in -80 °C freezer.

PCR

Steve and Thai

pIC691, pIC54

Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C. Products stored in -20 °C freezer.