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Team:Queens-Canada/10 June 2010
From 2010.igem.org
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==June 10, 2010== | ==June 10, 2010== | ||
| - | ====Gel==== | + | ====[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]==== |
Hao and Thai | Hao and Thai | ||
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Mistakes during gel loading are possible. | Mistakes during gel loading are possible. | ||
| - | ====Gel==== | + | ====[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]==== |
Hao and Thai | Hao and Thai | ||
'''pIC54''' and '''pIC691''' digestion products | '''pIC54''' and '''pIC691''' digestion products | ||
| - | ====Glycerol Stock==== | + | ====[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]==== |
Yuli | Yuli | ||
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Products stored in -80 °C freezer. | Products stored in -80 °C freezer. | ||
| - | ====PCR==== | + | ====[[Team:Queens-Canada/protocols#PCR|PCR]]==== |
Steve and Thai | Steve and Thai | ||
Revision as of 05:02, 27 October 2010
Contents |
June 10, 2010
Gel Electrophoresis
Hao and Thai
pSip-1, pUnc-47
Used 0.45g agarose in 65 mL TBE. Results: lanes 1,2,5, and 9 were visible. The rest of the gel was blank. The primers for unc-47 will definitely have to be replaced. Mistakes during gel loading are possible.
Gel Electrophoresis
Hao and Thai
pIC54 and pIC691 digestion products
Glycerol Stock
Yuli
pIC691 and pIC54
Change: 250 µL of 80% glycerol was used with 750 µL of culture.
Products stored in -80 °C freezer.
PCR
Steve and Thai
pIC691, pIC54
Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C. Products stored in -20 °C freezer.
