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Team:Queens-Canada/10 June 2010
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(New page: ==June 10, 2010== ====Gel==== Hao and Thai '''pSip-1''', '''pUnc-47''' Used 0.45g agarose in 65 mL TBE. Results: lanes 1,2,5, and 9 were visible. The rest of the gel was blank. The primer...) |
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====Gel==== | ====Gel==== | ||
Hao and Thai | Hao and Thai | ||
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'''pSip-1''', '''pUnc-47''' | '''pSip-1''', '''pUnc-47''' | ||
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====Gel==== | ====Gel==== | ||
Hao and Thai | Hao and Thai | ||
| + | |||
'''pIC54''' and '''pIC691''' digestion products | '''pIC54''' and '''pIC691''' digestion products | ||
====Glycerol Stock==== | ====Glycerol Stock==== | ||
Yuli | Yuli | ||
| + | |||
'''pIC691''' and '''pIC54''' | '''pIC691''' and '''pIC54''' | ||
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====PCR==== | ====PCR==== | ||
Steve and Thai | Steve and Thai | ||
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'''pIC691''', '''pIC54''' | '''pIC691''', '''pIC54''' | ||
Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C. | Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C. | ||
Products stored in -20 °C freezer. | Products stored in -20 °C freezer. | ||
Revision as of 04:46, 27 October 2010
Contents |
June 10, 2010
Gel
Hao and Thai
pSip-1, pUnc-47
Used 0.45g agarose in 65 mL TBE. Results: lanes 1,2,5, and 9 were visible. The rest of the gel was blank. The primers for unc-47 will definitely have to be replaced. Mistakes during gel loading are possible.
Gel
Hao and Thai
pIC54 and pIC691 digestion products
Glycerol Stock
Yuli
pIC691 and pIC54
Change: 250 µL of 80% glycerol was used with 750 µL of culture.
Products stored in -80 °C freezer.
PCR
Steve and Thai
pIC691, pIC54
Program run using TAQ with an extension time of 60 s and annealing temperature of 63 °C. Products stored in -20 °C freezer.
