Team:Peking/Notebook/XTeng

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   Xin Teng's Notes
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After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP.

There are many pictures and graphs in my notes, I strongly recommand you to download them for more precise reading.

download her notes


Contents

Contents

June

6.7-6.13

Primer design

  • For expression test

pET21a-MBP-His

pET 39b DsbA-MBP

pMAL p4X-MBP-MBP

pET21a-MerR-His

pET 39b DsbA-MerR

pMAL p4X-MBP-MerR

  • For standard part

MBP_SD

DsbA-MBP_SD

MBP-MBP_SD

MerR_SD

DsbA-MerR_SD

MBP-MerR_SD

6.14-6.20

Literature Review & Experimental Design for Periplasmic MBP


July