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Team:Peking/Notebook/XTeng
From 2010.igem.org
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After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP. | After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP. | ||
| + | There are many pictures and graphs in my notes, I strongly recommand you to download them for more precise reading. | ||
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| + | =='''Contents'''== | ||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ALiu#June| June, 2010]]</span> | ||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ALiu#July| July, 2010]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ALiu#August| August, 2010]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ALiu#September| September, 2010]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ALiu#October| October, 2010]]</span> | ||
| + | |||
| + | ==June== | ||
Revision as of 07:28, 26 October 2010
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Xin Teng's Notes
After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP.
There are many pictures and graphs in my notes, I strongly recommand you to download them for more precise reading.
Contents
June
