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      Vocabulary of Peking iGEM 2010



Reverse engineering

Reverse engineering is the process of discovering the technological principles of a device, object or system through analysis of its structure, function and operation. It often involves taking something apart and analyzing its workings in detail to be used in maintenance, or to try to make a new device or program that does the same thing without using or simply duplicating (without understanding) any part of the original. (Wikipedia)




MBP, a kind of mercury mental binding peptide that consists of two tandemed copies of metal binding domain of MerR including dimerization helix and metal binding loop together


DsbA (a periplasmic disulfide bond oxidoreductase),a SecA dependent signal protein , can export its C-terminal fusion protein into periplasm on the signal recognition particle (SRP) pathway


LOM (lpp-OmpA-MBP), a fusion protein consists of first 9 amino acids of lipoprotein, residues 46~159 of OmpA, an outer membrane protein and our lead binding peptide,MBP.

Traffic light bioassay

Traffic light bioassay, the assay we have designed utilized similar indications as road signal for directing vehicular traffic by means of colored lights when we expect that several shades of blue imply different concentrations of heavy metal ions detected.