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Horizontal Gene Transfer: What is going on?

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Horizontal gene transfer, the transfer of genetic materials between different species, gradually becomes a research hot spot over past decades. Although Genetic engineering microorganisms have sprung up in recent years and raised some safety issues when released, it does not necessarily indicate that HGT is good for nothing. In contrast, horizontal gene transfer has significant biological meaning. The evolution of species progresses rapidly not only by mutation and multiplication, but also by transfer of DNA.[1] Now it is believed that successful horizontal gene transfer could provide a selective advantage to either the host or the gene itself. [2] Does horizontal gene transfer happen often? Through conjugation, transformation and transduction, [3] any segment of DNA in a large bacterial population have opportunities to be horizontally transferred. However, the possibility of HGT is extremely low. Only a minor proportion of the DNA transferred between species is likely to be maintained in the new host over generations, and many factors influence horizontal gene acquisitions, including biological mechanistic barriers, temporal and spatial limitations.[1] Therefore, theoretic calculation and laboratory-observed HGT events are rarely linked to serious consequences. The relevant biosafety researches of many aspects could also help us evaluate the probability of horizontal gene transfer with rational criteria. From results and observations obtained under current studies, it can be concluded that there is little chance to cause hygiene problems and horizontal gene transfer in laboratory,[4] as long as principles of biological safety are well kept. [Table 1]

Nevertheless, from the investigation and interviews, a great many scientific researchers hardly learn national regulations of China, and the meaning of gene transfer, only knowing that many projects are hard to carry on or apply funding. (More details can be seen on the page of Special feature: HGT investigation) Almost all Professors complained about unfair treatments and law enforcement. Officials also confessed that regulations and laws on bio-safety, gene transfer and management system should be better formulated and implemented. The public tends to overreact to gene transfer could affect the development the genetic engineering industry and synthetic biological science, especially in developing countries, such as China who is just a beginner of synthetic biology. In a larger sense, the innovation room might have been narrowed by officials and public’s "crippling" over-concern with the potential abuse of -- not its effective use.

Of course, scientific development has not come without costs. Apart from the harmful byproducts such as prevalence of resistant gene and environmental destruction, it is worth noting that despite much focus on probability of HGT, effective estimates of HGT rates are hardly obtainable, so it is with the evaluation of long-term effects of HGT events.[8] Since rare HGT events could not be ignored within current understanding, people should have cautious consideration of HGT and careful use of GMO. Here’s a piece of good news is: horizontal gene transfer is detectable. [BOX 1] Usually, target cultures would be identified by PCR to detect specific sequences, fragment of characteristic protein, or examination of its restriction enzyme profile.[5] Again, overly concern is not necessary, when negative consequences would be avoided to a great extend if one does experiments and uses methods with great care and restraint. (Want to know more methods that could limit risks of HGT? see the page of PartII: Youth Team, Our Future)