Team:Peking

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
<!--Background-->
<!--Background-->
 +
{{:Team:Peking/BannerHidden}}
{{:Team:Peking/BannerHidden}}
 +
{{:Team:Peking/Background}}
{{:Team:Peking/Background}}
 +
<!--Header-->
<!--Header-->
 +
{{:Team:Peking/Header3}}
{{:Team:Peking/Header3}}
 +
 +
<!--Horizenal Navigation Menu-->
<!--Horizenal Navigation Menu-->
 +
<html>
<html>
 +
<table style="color:#EAC100;background:transparent;" cellpadding="3" cellspacing="1" border="0" width="80%" align="center">
<table style="color:#EAC100;background:transparent;" cellpadding="3" cellspacing="1" border="0" width="80%" align="center">
 +
<tr>
<tr>
 +
<th align="center"><strong class="selflink">Home</strong>
<th align="center"><strong class="selflink">Home</strong>
 +
</th><th align="center"><a href="/Team:Peking/Team" title="Team:Peking/Team">Team</a>
</th><th align="center"><a href="/Team:Peking/Team" title="Team:Peking/Team">Team</a>
 +
 +
</th><th align="center"><a href="/Team:Peking/Project" title="Team:Peking/Project">Project</a>
</th><th align="center"><a href="/Team:Peking/Project" title="Team:Peking/Project">Project</a>
 +
</th><th align="center"><a href="/Team:Peking/Parts" title="Team:Peking/Parts">Parts</a>
</th><th align="center"><a href="/Team:Peking/Parts" title="Team:Peking/Parts">Parts</a>
 +
</th><th align="center"><a href="/Team:Peking/Modeling" title="Team:Peking/Modeling">Modeling</a>
</th><th align="center"><a href="/Team:Peking/Modeling" title="Team:Peking/Modeling">Modeling</a>
 +
</th><th align="center"><a href="/Team:Peking/Notebook" title="Team:Peking/Notebook">Notebook</a>
</th><th align="center"><a href="/Team:Peking/Notebook" title="Team:Peking/Notebook">Notebook</a>
 +
</th><th align="center"><a href="/Team:Peking/Human_Practice" title="Team:Peking/Human Practice">Human Practice</a>
</th><th align="center"><a href="/Team:Peking/Human_Practice" title="Team:Peking/Human Practice">Human Practice</a>
 +
</th></tr></table>
</th></tr></table>
Line 22: Line 40:
</html>
</html>
 +
 +
 +
<!--sideBar-->
<!--sideBar-->
 +
{{:Team:Peking/SideBar}}
{{:Team:Peking/SideBar}}
 +
 +
<!--Main Content-->
<!--Main Content-->
 +
<html>
<html>
-
<head>
+
 
 +
 +
 
 +
<div id="content">
 +
 
 +
 
 +
 
 +
 
<style type="text/css">
<style type="text/css">
 +
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}
.b1f, .b2f, .b3f, .b4f{font-size:1px; overflow:hidden; display:block;}
 +
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}
.b1f {height:1px; background:#e1e1e1; margin:0 5px;}
 +
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}
.b2f {height:1px; background:#e1e1e1; margin:0 3px;}
 +
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}
.b3f {height:1px; background:#e1e1e1; margin:0 2px;}
 +
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}
.b4f {height:2px; background:#e1e1e1; margin:0 1px;}
 +
.content {background: #e1e1e1;}
.content {background: #e1e1e1;}
 +
.content div {margin-left: 5px;}
.content div {margin-left: 5px;}
 +
</style>
</style>
-
</head>
 
-
<div class="all">
 
<table width=60% style="background:transparent;">
<table width=60% style="background:transparent;">
 +
<tr>
<tr>
 +
<td style="height: 200; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
<td style="height: 200; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
 +
     <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>   
     <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>   
 +
<!--to make the split line-->
<!--to make the split line-->
 +
     <div class="Recoli">
     <div class="Recoli">
 +
     <div style="height: 400; background:transparent;">
     <div style="height: 400; background:transparent;">
 +
     <h1><b><font color="b7ff4a"><font face="Showcard Gothic">Heavy Metal Decontamination Kit</font></font></b></h1>
     <h1><b><font color="b7ff4a"><font face="Showcard Gothic">Heavy Metal Decontamination Kit</font></font></b></h1>
 +
 +
<img src="https://static.igem.org/mediawiki/2010/3/32/Project_pic.jpg" width="550px" alt="pic down">
<img src="https://static.igem.org/mediawiki/2010/3/32/Project_pic.jpg" width="550px" alt="pic down">
 +
 +
<div align="justify">
<div align="justify">
 +
<font size="2"><font face="Comic Sans MS"><font color="eac100">
<font size="2"><font face="Comic Sans MS"><font color="eac100">
 +
<p>In the field of biodetection and bioremediation, despite numerous proofs of principle, most bioreporters and bioabsorbents have remained confined to the laboratory. Also bacterial reporters and absorbents have NOT been rational enough in design and NOT complex enough in function. Additionally, we notice that genetic engineering in this field needs streamline methods and it's time for a series of issues on the field application to be taken into consideration.</p>
<p>In the field of biodetection and bioremediation, despite numerous proofs of principle, most bioreporters and bioabsorbents have remained confined to the laboratory. Also bacterial reporters and absorbents have NOT been rational enough in design and NOT complex enough in function. Additionally, we notice that genetic engineering in this field needs streamline methods and it's time for a series of issues on the field application to be taken into consideration.</p>
 +
<p>In this summer, we engineered our bacteria to resolve those hard truths mentioned above. MerR family transcription factors (TFs) was exploited to construct a series of biorepoters for heavy metal detection and bioabsorbents for heavy metal decontamination, based on Reverse Engineering principle. </p>
<p>In this summer, we engineered our bacteria to resolve those hard truths mentioned above. MerR family transcription factors (TFs) was exploited to construct a series of biorepoters for heavy metal detection and bioabsorbents for heavy metal decontamination, based on Reverse Engineering principle. </p>
 +
<p>MerR is a mercury responsive regulator, a representative of MerR family TFs. Primarily, we analyzed function and operation of a MerR in detail via bioware experiments. Then modeling was conducted to analyze the characteristics of MerR and the topology of its regulation behavior. Knowledge from the analyses enabled us to design genetic circuit that makes mercury sensor and regulator components more efficient and robuster. Our engineered bioreporters are able to discriminate concentration ranges of certain heavy metal in water independently of incubation time and cell activity in a wide window. This means that the field application can be carried out without the need for costly equipment while response quality and sensor sensitivity are still kept. Secondly, we constructed bioabsorbent strains that decontaminate mercury from polluted aquatic environments. In our bioabsorbents, MerR was engineered into a single-chain, antiparallel, coiled coil peptide that mimics the metal binding domains, which reduces cost of metal binding. This was followed by inductive expression of engineered MerR protein on surface, periplasm and cytosol of E.coli cells. Mercury (II) absorption test showed that our bacteria can absorb more than 95% of 10^-7M Hg(II) in 5 minutes. </p>
<p>MerR is a mercury responsive regulator, a representative of MerR family TFs. Primarily, we analyzed function and operation of a MerR in detail via bioware experiments. Then modeling was conducted to analyze the characteristics of MerR and the topology of its regulation behavior. Knowledge from the analyses enabled us to design genetic circuit that makes mercury sensor and regulator components more efficient and robuster. Our engineered bioreporters are able to discriminate concentration ranges of certain heavy metal in water independently of incubation time and cell activity in a wide window. This means that the field application can be carried out without the need for costly equipment while response quality and sensor sensitivity are still kept. Secondly, we constructed bioabsorbent strains that decontaminate mercury from polluted aquatic environments. In our bioabsorbents, MerR was engineered into a single-chain, antiparallel, coiled coil peptide that mimics the metal binding domains, which reduces cost of metal binding. This was followed by inductive expression of engineered MerR protein on surface, periplasm and cytosol of E.coli cells. Mercury (II) absorption test showed that our bacteria can absorb more than 95% of 10^-7M Hg(II) in 5 minutes. </p>
 +
<p>MerR family TFs share high homology at their metal binding domains, which implies that our strategies of bioreporter and bioabsorbent engineering might be applicable to other cases. We then expanded our reverse engineering strategy to another common toxic heavy metal, lead. A series of function tests showed that the lead bioreporters and bioabsorbents are comparable to mercury ones, proving validness of our engineering strategy. As almost each species of heavy metal has a corresponding MerR family TF, we can state that we have developed a streamlined method to construct heavy metal decontamination kits composed of valid bioreporters and bioabsorbents for field application.</p>
<p>MerR family TFs share high homology at their metal binding domains, which implies that our strategies of bioreporter and bioabsorbent engineering might be applicable to other cases. We then expanded our reverse engineering strategy to another common toxic heavy metal, lead. A series of function tests showed that the lead bioreporters and bioabsorbents are comparable to mercury ones, proving validness of our engineering strategy. As almost each species of heavy metal has a corresponding MerR family TF, we can state that we have developed a streamlined method to construct heavy metal decontamination kits composed of valid bioreporters and bioabsorbents for field application.</p>
 +
 +
</font></font></font></div>
</font></font></font></div>
 +
 +
 +
       </div></div>
       </div></div>
 +
 +
 +
 +
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
 +
     </td>
     </td>
 +
   </tr>
   </tr>
 +
 +
<tr>
<tr>
 +
<td style="height: 800; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
<td style="height: 800; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
 +
     <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
     <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
 +
     <div class="Overview">
     <div class="Overview">
 +
     <div style="height: 800; background:transparent;">
     <div style="height: 800; background:transparent;">
 +
     <h2></h2>
     <h2></h2>
 +
 +
<div align="justify">
<div align="justify">
 +
<font size="2">
<font size="2">
 +
<p></p>
<p></p>
 +
 +
<br>
<br>
 +
<br>
<br>
 +
<br>
<br>
-
 
</font>
</font>
 +
</div>
</div>
 +
       </div></div>
       </div></div>
-
 
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
 +
 +
     </td>
     </td>
 +
   </tr>
   </tr>
 +
<tr>
<tr>
 +
<td style="height: 800; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
<td style="height: 800; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
 +
     <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
     <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
 +
     <div class="Overview">
     <div class="Overview">
 +
     <div style="height: 800; background:transparent;">
     <div style="height: 800; background:transparent;">
 +
     <h2></h2>
     <h2></h2>
 +
 +
<div align="justify">
<div align="justify">
 +
<font size="2">
<font size="2">
 +
<P> </p>
<P> </p>
 +
 +
<br>
<br>
 +
<br>
<br>
 +
<br>
<br>
 +
</font>
</font>
 +
</div>
</div>
 +
       </div></div>
       </div></div>
Line 124: Line 224:
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
 +
 +
     </td>
     </td>
 +
   </tr>
   </tr>
 +
</table>
</table>
 +
</div>
</div>
 +
</div>
-
</HTML>
 
 +
 +
 +
 +
 +
<div class="all">
 +
 +
 +
 +
</html>
<!--foot-->
<!--foot-->

Revision as of 15:57, 16 September 2010






Home Team Project Parts Modeling Notebook Human Practice








Heavy Metal Decontamination Kit

pic down

In the field of biodetection and bioremediation, despite numerous proofs of principle, most bioreporters and bioabsorbents have remained confined to the laboratory. Also bacterial reporters and absorbents have NOT been rational enough in design and NOT complex enough in function. Additionally, we notice that genetic engineering in this field needs streamline methods and it's time for a series of issues on the field application to be taken into consideration.

In this summer, we engineered our bacteria to resolve those hard truths mentioned above. MerR family transcription factors (TFs) was exploited to construct a series of biorepoters for heavy metal detection and bioabsorbents for heavy metal decontamination, based on Reverse Engineering principle.

MerR is a mercury responsive regulator, a representative of MerR family TFs. Primarily, we analyzed function and operation of a MerR in detail via bioware experiments. Then modeling was conducted to analyze the characteristics of MerR and the topology of its regulation behavior. Knowledge from the analyses enabled us to design genetic circuit that makes mercury sensor and regulator components more efficient and robuster. Our engineered bioreporters are able to discriminate concentration ranges of certain heavy metal in water independently of incubation time and cell activity in a wide window. This means that the field application can be carried out without the need for costly equipment while response quality and sensor sensitivity are still kept. Secondly, we constructed bioabsorbent strains that decontaminate mercury from polluted aquatic environments. In our bioabsorbents, MerR was engineered into a single-chain, antiparallel, coiled coil peptide that mimics the metal binding domains, which reduces cost of metal binding. This was followed by inductive expression of engineered MerR protein on surface, periplasm and cytosol of E.coli cells. Mercury (II) absorption test showed that our bacteria can absorb more than 95% of 10^-7M Hg(II) in 5 minutes.

MerR family TFs share high homology at their metal binding domains, which implies that our strategies of bioreporter and bioabsorbent engineering might be applicable to other cases. We then expanded our reverse engineering strategy to another common toxic heavy metal, lead. A series of function tests showed that the lead bioreporters and bioabsorbents are comparable to mercury ones, proving validness of our engineering strategy. As almost each species of heavy metal has a corresponding MerR family TF, we can state that we have developed a streamlined method to construct heavy metal decontamination kits composed of valid bioreporters and bioabsorbents for field application.