Team:Peking

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{| style="color:00009f;background-color:ffffff;" cellpadding="3" cellspacing="1" border="0" bordercolor="white" width="80%" align="center"
 
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!align="center"|[[Team:Peking|Home]]
 
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!align="center"|[[Team:Peking/Team|Team]]
 
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!align="center"|[[Team:Peking/Project|Project]]
 
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!align="center"|[[Team:Peking/Parts|Parts]]
 
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    <h1><b><font color="00009f"><font face="Showcard Gothic">Project Description</font></font></b></h1>
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<p>In the field of biodetection and bioremediation, despite numerous proofs of principle, most bioreporters and bioabsorbents have remained confined to the laboratory. Also bacterial reporters and absorbents have NOT been rational enough in design and NOT complex enough in function. Additionally, we notice that genetic engineering in this field needs streamline methods and it's time for a series of issues on the field application to be taken into consideration.</p>
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<p>In this summer, we engineered our bacteria to resolve those hard truths mentioned above. MerR family transcription factors (TFs) was exploited to construct a series of biorepoters for heavy metal detection and bioabsorbents for heavy metal decontamination, based on Reverse Engineering principle. </p>
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<p>MerR is a mercury responsive regulator, a representative of MerR family TFs. Primarily, we analyzed function and operation of a MerR in detail. Then modeling was conducted to analyze the characteristics of MerR and the topology of its regulation behavior. Knowledge from the analyses enabled us to design genetic circuit that makes mercury sensor and regulator components more efficient and robuster. Our engineered bioreporters are able to discriminate concentration ranges of certain heavy metal in water independently of incubation time and cell activity in a wide window. This means that the field application can be carried out without the need for costly equipment while response quality and sensor sensitivity are still kept. Secondly, we constructed bioabsorbent strains that decontaminate mercury from polluted water. In our bioabsorbents, MerR was engineered into a single-chain, antiparallel, coiled coil peptide that mimics the metal binding domains, which reduces cost of metal binding. This was followed by inductive expression of engineered MerR protein on surface, periplasm and cytosol of E.coli cells. Mercury (II) absorption test showed that our bacteria can absorb more than 95% of 10^-7M Hg(II) in 5 minutes. </p>
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<p>MerR family TFs share high homology at their metal binding domains, which implies that our strategies of bioreporter and bioabsorbent engineering might be applicable to other cases. We then expanded our reverse engineering strategy to another common toxic heavy metal, lead. A series of function tests showed that the lead bioreporters and bioabsorbents are comparable to mercury ones, proving validness of our engineering strategy. As almost each species of heavy metal has a corresponding MerR family TF, we can state that we have developed a streamlined method to construct heavy metal decontamination kits composed of valid bioreporters and bioabsorbents for field application.</p>
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<a class="B1" a href="http://2010.igem.org/Team:Peking/Project/Biosensor">Biosensor<img src="http://2010.igem.org/wiki/images/1/1b/Sensorintro.png"/></a>
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<a class="B2" a href="http://2010.igem.org/Team:Peking/Project/Bioabsorbent">Bioabsorbent<img src="http://2010.igem.org/wiki/images/8/84/Absorbentintro.png"/></a>
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<a class="B3" a href="http://2010.igem.org/Team:Peking/Project/Application">Application<img src="http://2010.igem.org/wiki/images/b/b6/Applicationintro.png"/></a>
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<a class="B4" a href="http://2010.igem.org/Team:Peking/Project/Expansion">Expansion<img src="http://2010.igem.org/wiki/images/b/b1/Expansionintro.png"/></a>
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==Parts==
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<html><a href="http://2010.igem.org/Team:Peking/Parts"><img src="http://2010.igem.org/wiki/images/d/da/Pku_part3.jpg" width=350 height=135></a></html>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a expansible method for heavy metal bioreporter and bioabsorbent construction.</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <html><a href="http://2010.igem.org/Team:Peking/Parts"><font size=4>learn more</font></a></p></html>
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==Team==
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<html><a href="http://2010.igem.org/Team:Peking/Team"> <img src="http://2010.igem.org/wiki/images/c/c2/TeamPKUPp.jpg" width=510></a></html>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;Eighteen students and four instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<html><a href="http://2010.igem.org/Team:Peking/Team"><font size=4>learn more</font></a></p></html>
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==Modeling==
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<html><a href="http://2010.igem.org/Team:Peking/Modeling"><img src="http://2010.igem.org/wiki/images/7/76/Modelingpku.jpg" width=500 height=230 alt="Modeling"  ></a>
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&nbsp;&nbsp;&nbsp;&nbsp;We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://2010.igem.org/Team:Peking/Modeling"><font size=4>learn more</font></a>
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==Human Practice==
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<html><a href="http://2010.igem.org/Team:Peking/Humanpractice"><img src="http://2010.igem.org/wiki/images/8/84/FrontpageHP.jpg" width=350 height=127 alt="Biosafety"  align="right"></a>
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&nbsp;&nbsp;&nbsp;&nbsp;DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner. <br>
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&nbsp;&nbsp;&nbsp;&nbsp; This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, and raising the awareness of researchers on such issues.<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://2010.igem.org/Team:Peking/Humanpractice"><font size=4>learn more</font></a>
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==Gallery==
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<p>&nbsp;&nbsp;&nbsp;&nbsp;It's an unforgettable summer, during which we spared no effort to fight for our dreams and the honour of Peking iGEM team. Photos recorded every footprint of everyone. Join us together!</p>
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<img src="http://2010.igem.org/wiki/images/2/2d/PKU_IGEM20102.gif" width="100px" alt="the whole team" >&nbsp;
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&nbsp;<img src="http://2010.igem.org/wiki/images/2/2f/%E5%9B%BE%E5%83%8F0201.jpg" width="100px" alt="Balloon on the experiment table" width="50px" >&nbsp;&nbsp;<img src="http://2010.igem.org/wiki/images/0/03/Rats.gif" width="100px" alt="Team's mascot~" >&nbsp;&nbsp;<img src="http://2010.igem.org/wiki/images/4/46/Assembled.jpg" width="100px" alt="Our lab">&nbsp;&nbsp;<img src="http://2010.igem.org/wiki/images/8/88/P_large_oReI_3ff9000598fc2d11.jpg" width="115px" alt="Ao Liu & Ying Shen's birthday party">&nbsp;&nbsp;<img src="http://2010.igem.org/wiki/images/1/1c/P_large_MA7H_33dd00001e802d0b.jpg" width="230px" alt="Weekly Meeting"></a> &nbsp;&nbsp;<img src="http://2010.igem.org/wiki/images/a/ab/Pkupuzzle521.jpg" width="100px" alt="Team's pyjama~"></a></html>
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Latest revision as of 22:17, 27 October 2010

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Parts

    Our team submitted a library of thoroughly characterized and standardized parts. Therefore contributing an alternative set of tools towards heavy metal detection and decontamination in the iGEM context. What's more, as our MerR-family-derivated engineering method could be applicable in nearly all cases of heavy metal, our parts and corresponding documents provide a expansible method for heavy metal bioreporter and bioabsorbent construction.

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Team

    Eighteen students and four instructors are working on our project during this summer. We split up into several subgroups whose focus and results you can follow on the Notebook or Project pages. If you want to know more about the subgroups and the people involved, meet us on our Team page.


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Modeling

Modeling     We adopt the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analysis whether they fit the objection function or not, thus getting the right topology and the proper range of parameters for the designation of our project.
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Human Practice

Biosafety     DNA recombination, Fragment synthesis, Bio-security... these are words that could hardly be ignored when doing researches of synthetic biology, which promises a great many improvements to our understanding of this world. The potential bio-security risk, however, is just around corner.
     This summer, we did a thorough research on this topic, aiming at proactively finding out the current situation in China, and raising the awareness of researchers on such issues.
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