"

From 2010.igem.org

• Construction of our counters

First of all, we spent a significant part of the summer to do cloning. For this, we have used a lot of biobricks submitted to the parts registry by previous teams, thank you! Using these parts we have created plenty of new composite parts that are highly modular (different kind of RBS and promoters) and could serve next iGEM teams. We have also created some new biobricks that are at the core of our project. We hope other iGEM teams will work with integrons and might need these parts.
Moreover, each of our constructs was verified by sequencing. For more information you can take a look at the parts that we sent to the registry.

• Check if IntI1 integrase works

5h, 6h, 7h, 8h

• Calculate the recombination rate

• Check the excision of the terminator by sequencing

• Verify the toxicity of the integrase

• Test the microfluidic device