Team:Paris Liliane Bettencourt/Project/Population counter/Results

From 2010.igem.org

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Revision as of 19:26, 27 October 2010




  • Construction of our counters


First of all, we spent a significant part of the summer to do cloning. For this, we have used a lot of biobricks submitted to the parts registry by previous teams, thank you! Using these parts we have created plenty of new composite parts that are highly modular (different kind of RBS and promoters) and could serve next iGEM teams. We have also created some new biobricks that are at the core of our project. We hope other iGEM teams will work with integrons and might need these parts.
Moreover, each of our constructs was verified by sequencing. For more information you can take a look at the parts that we sent to the registry.

  • Check if IntI1 integrase works


Ara1 1.jpg Ara1 2.jpg Ara1 3.jpg Ara1 4.jpg
5h, 6h, 7h, 8h
Ara1 5.jpg Ara1 6.jpg Ara1 7.jpg Ara1 8.jpg

  • Calculate the recombination rate
Recombination rate.jpg

Tetracyclin resistance.jpg



  • Check the excision of the terminator by sequencing



  • Verify the toxicity of the integrase

IntI1 toxicity.jpg



  • Test the microfluidic device