Minipreps

• sLac/LuxI III
• LuxI
• RBS/GFP/tt (E0240)
• pLux
• Pms/mTet/LuxI
• I13507/pLux/GFP
• PswTet, colonies 1 and 2
• PsmTet, colonies 1 and 2
• PssTet, colonies 1 and 2

Restriction digest

With XbaI and PstI

• LuxI
• I13507/pLux/GFP

With EcoRI-HF and XbaI

• RBS/GFP/tt (E0240)

With SpeI and PstI

• pLux
• Pms/mTet/LuxI

With EcoRI-HF and SpeI

• LuxI

With EcoRI-HF and PstI

• PswTet, colonies 1 and 2
• PsmTet, colonies 1 and 2
• PssTet, colonies 1 and 2

Gel electrophoresis

• LuxI (X+P)
• LuxI (E+S)
• I13507/pLux/GFP
• PswTet, colonies 1
• PswTet, colonies 2
• PsmTet, colonies 1
• PsmTet, colonies 2
• PssTet, colonies 1
• PssTet, colonies 2

Gel extraction

• LuxI (X+P)
• LuxI (E+S)
• I13507/pLux/GFP

PCR purification

• RBS/GFP/tt (E0240)
• pLux
• Pms/mTet/LuxI