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Colony PCR
Gradient PCR (53-73°C to determine hybridization temperature of VF2+VR)
Primers : VF2 + VR

• 12x PmS-WTet III
• 12x PsM-MTet III

Minipreps

• WLac-LuxI II
• WLac-LuxI III
• SLac-LuxI II
• SLac-LuxI III
• pLux-LuxI II
• pLux-LuxI III
• PsS-MTet II
• PsS-MTet III
• PmS-WTet II
• PmS-WTet III
• PsM-MTet II
• PsM-MTet III
• PsW-MTet II
• PsW-MTet III
• PsW-WTet II
• PsW-WTet III
• PsM-WTet II
• PsM-WTet III
• PsS-WTet II
• PsS-WTet III

Restriction digest with SpeI and EcoRI-HF

• pLux-LuxI II
• pLux-LuxI III
• PsS-MTet II
• PsS-MTet III
• PmS-WTet II
• PmS-WTet III
• PsM-MTet II
• PsM-MTet III
• PsW-MTet II
• PsW-MTet III
• PsW-WTet II
• PsW-WTet III
• PsM-WTet II
• PsM-WTet III
• PsS-WTet II
• PsS-WTet III

Restriction digest with XbaI and PstI

• WLac-LuxI II
• WLac-LuxI III
• SLac-LuxI II
• SLac-LuxI III

Gel electrophoresis

• pLux-LuxI II
• Ladder 1Kb
• pLux-LuxI III
• -
• PsS-MTet II
• -
• PsS-MTet III
• -
• PmS-WTet II
• -
• PmS-WTet III
• -
• PsM-MTet II
• -
• PsM-MTet III
• -
• PsW-MTet II

Gel electrophoresis

• PsW-MTet III
• -
• PsW-WTet II
• -
• PsW-WTet III
• -
• PsM-WTet II
• -
• PsM-WTet III
• -
• PsS-WTet II
• -
• PsS-WTet III
• Ladder 1Kb
• -
• WLac-LuxI II

Gel electrophoresis

• Ladder 1Kb
• WLac-LuxI III
• -
• SLac-LuxI II
• -
• SLac-LuxI III

Overnight cultures

• Ps/wRBS/LuxR/attC/term/attC/mRFP
• Ps/mRBS/LuxR/attC/term/attC/mRFP
• Ps/sRBS/LuxR/attC/term/attC/mRFP
• Pm/sRBS/LuxR/attC/term/attC/mRFP
• wRBS/TetA(C)/LuxI
• mRBS/TetA(C)/LuxI
• sRBS/TetA(C)/LuxI
• wRBS/LacZα
• sRBS/LacZα
• LuxI
• J13507/pLux/GFP
• pLux
• attP (in CmR)