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</td></tr> <tr> <td style="background-color: #EEE"><a href="/wiki/Image:Igem-logo-150px.png" class="image" title="Igem-logo-150px.png"><img alt="" src="/images/7/7b/Igem-logo-150px.png" width="150" height="115" border="0" /></a>Population counter </td><td style="background-color: #F2F2F2" align="center"><img src="/images/9/94/Report.png" border="0" /> <a href="/wiki/IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010" title="IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010">Main project page</a>
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Raphaël
Colony PCR
Touchdown PCR, 2µL of overnight culture
Primers : VF2 + VR

• WLac-LuxI I
• WLac-LuxI II
• WLac-LuxI III
• WLac-LuxI IV
• SLac-LuxI I
• SLac-LuxI II
• SLac-LuxI III
• pLux-LuxI I
• pLux-LuxI II
• pLux-LuxI III
• pLux-LuxI IV
• PsS-MTet I
• PsS-MTet II
• PsS-MTet III
• PsS-MTet IV
• PmS-WTet I
• PmS-WTet II
• PmS-WTet III
• PmS-WTet IV
• PsM-MTet I
• PsM-MTet II
• PsM-MTet III
• PsM-MTet IV
• PsW-MTet I
• PsW-MTet II
• PsW-MTet III
• PsW-MTet IV
• PsW-WTet I
• PsW-WTet II
• PsW-WTet III
• PsW-WTet IV
• PsM-WTet I
• PsM-WTet II
• PsM-WTet III
• PsM-WTet IV
• PsS-WTet II
• PsS-WTet III
• PsS-WTet IV

POWER ERROR after 30 cycles !

Gel electrophoresis

• WLac-LuxI I
• WLac-LuxI II
• WLac-LuxI III
• WLac-LuxI IV
• SLac-LuxI I
• SLac-LuxI II
• SLac-LuxI III
• pLux-LuxI I
• pLux-LuxI II
• pLux-LuxI III
• pLux-LuxI IV
• PsS-MTet I
• PsS-MTet II
• PsS-MTet III
• PsS-MTet IV
• PmS-WTet I
• Ladder 1Kb

<a href="/wiki/Image:Gel2908_1.jpg" class="image" title="Image:gel2908_1.jpg"><img alt="Image:gel2908_1.jpg" src="/images/d/d2/Gel2908_1.jpg" width="768" height="574" border="0" /></a>

Gel electrophoresis

• PmS-WTet II
• Ladder 1Kb
• PmS-WTet III
• PmS-WTet IV
• PsM-MTet I
• PsM-MTet II
• PsM-MTet III
• PsM-MTet IV
• PsW-MTet I
• PsW-MTet II
• PsW-MTet III
• PsW-MTet IV
• PsW-WTet I
• PsW-WTet II
• PsW-WTet III
• PsW-WTet IV
• PsM-WTet I

<a href="/wiki/Image:Gel2908_2.jpg" class="image" title="Image:gel2908_2.jpg"><img alt="Image:gel2908_2.jpg" src="/images/7/71/Gel2908_2.jpg" width="768" height="574" border="0" /></a>

Gel electrophoresis

• PsM-WTet II
• PsM-WTet III
• PsM-WTet IV
• PsS-WTet II
• PsS-WTet III
• PsS-WTet IV
• Ladder 1Kb

<a href="/wiki/Image:Gel2908_3.jpg" class="image" title="Image:gel2908_3.jpg"><img alt="Image:gel2908_3.jpg" src="/images/d/dd/Gel2908_3.jpg" width="768" height="574" border="0" /></a>

Overnight cultures

1/50 dilution from yesterday's overnight cultures

• WLac-LuxI I
• WLac-LuxI II
• WLac-LuxI III
• WLac-LuxI IV
• SLac-LuxI I
• SLac-LuxI II
• SLac-LuxI III
• pLux-LuxI I
• pLux-LuxI II
• pLux-LuxI III
• pLux-LuxI IV
• PsS-MTet I
• PsS-MTet II
• PsS-MTet III
• PsS-MTet IV
• PmS-WTet I
• PmS-WTet II
• PmS-WTet III
• PmS-WTet IV
• PsM-MTet I
• PsM-MTet II
• PsM-MTet III
• PsM-MTet IV
• PsW-MTet I
• PsW-MTet II
• PsW-MTet III
• PsW-MTet IV
• PsW-WTet I
• PsW-WTet II
• PsW-WTet III
• PsW-WTet IV
• PsM-WTet I
• PsM-WTet II
• PsM-WTet III
• PsM-WTet IV
• PsS-WTet II
• PsS-WTet III
• PsS-WTet IV