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Raphaël and Aleksandra
Glycerols, 15%

• mRBS/TetA(C)+RBS/LuxI, 4 colonies
• wRBS/TetA(C)+RBS/LuxI, 4 colonies
• stRBS/TetA(C)+RBS/LuxI, 4 colonies
• RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies
• RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies
• Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies
• Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies
• Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony
• Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies

Léa and Théotime
Minipreps of 10ml, elution in 35µl warm water

• mRBS/TetA(C)+RBS/LuxI, 4 colonies
• wRBS/TetA(C)+RBS/LuxI, 4 colonies
• stRBS/TetA(C)+RBS/LuxI, 4 colonies
• RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies
• RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies
• Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies
• Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies
• Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony
• Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies

Restriction digest

• mRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• wRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• stRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
• Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes

Gel electrophoresis, 1.5%agarose, for verification only
Gel 1

• mRBS/TetA(C)+RBS/LuxI col1
• mRBS/TetA(C)+RBS/LuxI col2
• mRBS/TetA(C)+RBS/LuxI col3
• mRBS/TetA(C)+RBS/LuxI col4
• wRBS/TetA(C)+RBS/LuxI col1
• wRBS/TetA(C)+RBS/LuxI col2
• wRBS/TetA(C)+RBS/LuxI col3
• wRBS/TetA(C)+RBS/LuxI col4
• stRBS/TetA(C)+RBS/LuxI col1
• stRBS/TetA(C)+RBS/LuxI col2
• stRBS/TetA(C)+RBS/LuxI col3
• stRBS/TetA(C)+RBS/LuxI col4
• RBS/mRFP/t(K081014)+pLux/GFP col1
• RBS/mRFP/t(K081014)+pLux/GFP col2
• RBS/mRFP/t(K081014)+pLux/GFP col3
• RBS/mRFP/t(K081014)+pLux/GFP col4

<a href="/index.php?title=Special:Upload&wpDestFile=Gel1208_1.jpg" class="new" title="Image:Gel1208 1.jpg">Image:Gel1208 1.jpg</a>

Gel 2

• RBS/mRFP/tt(I13507)+pLux/GFP col1
• RBS/mRFP/tt(I13507)+pLux/GFP col2
• RBS/mRFP/tt(I13507)+pLux/GFP col3
• RBS/mRFP/tt(I13507)+pLux/GFP col4
• Ps/wRBS/LuxR +attC/term/attC/mRFP col1
• Ps/wRBS/LuxR +attC/term/attC/mRFP col2
• Ps/wRBS/LuxR +attC/term/attC/mRFP col3
• Ps/mRBS/LuxR +attC/term/attC/mRFP col1
• Ps/mRBS/LuxR +attC/term/attC/mRFP col2
• Ps/mRBS/LuxR +attC/term/attC/mRFP col3
• Ps/mRBS/LuxR +attC/term/attC/mRFP col4
• Pm/sRBS/LuxR +attC/term/attC/mRFP
• Ps/sRBS/LuxR +attC/term/attC/mRFP col1
• Ps/sRBS/LuxR +attC/term/attC/mRFP col2
• Ps/sRBS/LuxR +attC/term/attC/mRFP col3
• Ps/sRBS/LuxR +attC/term/attC/mRFP col4