Team:Paris Liliane Bettencourt/Notebook/2010/08/03/

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Théotime and Raphaël
Transformation of TURBO cells, incubation at 37°C around 10h

  • RBS/LuxI/term (ligation of 26/07), with 50µl
  • RBS/LuxI/term (ligation of 26/07), with 100µl
  • positive control


Aleksandra
Minipreps of 10ml, elution in 35µl warm water

  • attC (from glycerol stock)
  • term/attC/mRFP (from glycerol stock)
  • attC/term/attC/mRFP #10 (from yesterday's glycerol stock)
  • attC/term/attC/mRFP #11 (from yesterday's glycerol stock)


DNA quantification in the minipreps using the nanodrop

Sample name 260/280 260/230 ng/µl
attC 2.13 1.69 135.2
term2.13 2.07169.9
attC/term/attC/mRFP #10 2.021.91 118.0