Team:Paris Liliane Bettencourt/Notebook/2010/08/01/

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Aleksandra
Glycerols of the yesterday's overnight cultures
15%glycerol (375µl 60%glycerol + 1125µl cultute), 2 for each (different colonies):

  • attC/term/attC/mRFP
  • weak RBS/TetA(C)/LuxI/term
  • medium RBS/TetA(C)/LuxI/term
  • strong RBS/TetA(C)/LuxI/term
  • weak RBS/LacZ alpha/LuxI/term
  • strong RBS/LacZ alpha/LuxI/term



Minipreps of the yesterday's overnight cultures, 2 for each part(cultures starting from colonies #1 and #2 on each plate)
Elution in 35µl EB buffer

  • attC/term/attC/mRFP
  • weak RBS/TetA(C)/LuxI/term
  • medium RBS/TetA(C)/LuxI/term
  • strong RBS/TetA(C)/LuxI/term
  • weak RBS/LacZ alpha/LuxI/term
  • strong RBS/LacZ alpha/LuxI/term


Quantification of the DNA in the minipreps by Nanodrop

Sample name260/280260/230ng/µl
attC/term/attC/mRFP 11.97 2.11 159.6
attC/term/attC/mRFP 21.96 1.87 195.3
weakRBS/TetA(C)/LuxI/term 11.96 2.28381.3
weakRBS/TetA(C)/LuxI/term 21.96 2.30291.9
mediumRBS/TetA(C)/LuxI/term 11.96 2.26 353.2
mediumRBS/TetA(C)/LuxI/term 2 1.95 2.28490.1
standardRBS/TetA(C)/LuxI/term 1 1.962.27 425.2
standardRBS/TetA(C)/LuxI/term 21.96 2.31 362.6
weakRBS/LacZ alpha/LuxI/term 1 1.96 2.20 260.2
weakRBS/LacZ alpha/LuxI/term 2 1.97 2.28 273.3
standardRBS/LacZ alpha/LuxI/term 1 1.982.24 248.6
standardRBS/LacZ alpha/LuxI/term 2 1.97 2.23265.0
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