Team:Paris Liliane Bettencourt/Notebook/2010/07/30/

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Aleksandra
Gel purification with the Quiagen kit
Purification of the bands extracted yesterday :

  • term/attC/mRFP
  • weak.RBS /TetA(C)
  • med.RBS /TetA(C)
  • stand.RBS /TetA(C)
  • weak.RBS /LacZ
  • stand.RBS /LacZ


Léa
PCR purification with the Quiagen kit

  • attC
  • LuxI/term


Léa, Aleksandra and Raphaël
Nanodrop
Quantification of DNA amounts in the minipreps from yesterday

Sample name260/280260/230ng/µl
stand.RBS /TetA(C) 1 1.93 2.31 271.3
pLux/RBS/GFP/term A 1.90 2.18 244.8
Pmed/weakRBS/LuxR 1 1.91 1.52 87.8
Pstr/weakRBS/LuxR 1 1.94 2.28 88.4
Pmed /medRBS/LuxR 1 1.89 2.19 156.2
Pstr/medRBS/LuxR 1 1.91 2.04 83.9
Pmed/strRBS/LuxR 1 1.94 2.37 236.5
Pstr/strRBS/LuxR 1 1.96 2.43 101.3
stand.RBS/LacZ 1 1.88 1.68 224.7
weak RBS/LacZ 1 1.92 2.06 162.4
LuxI/term 1 1.91 2.18 325.2
attC 1.97 1.96 152.0
term/attC/mRFP 2.03 2.36 213.2