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Theotime: New page: {{Template:paris2010_2}} <p style="display:block;"><font color="red">Antoine</font> </p><p style="display:block;"><br /><u>Résults transformation biobrick R0040/C0040/R0011/C0012</u> Po...
2010-10-25T20:09:19Z
<p>New page: {{Template:paris2010_2}} <p style="display:block;"><font color="red">Antoine</font> </p><p style="display:block;"><br /><u>Résults transformation biobrick R0040/C0040/R0011/C0012</u> Po...</p>
<p><b>New page</b></p><div>{{Template:paris2010_2}}<br />
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<p style="display:block;"><font color="red">Antoine</font><br />
</p><p style="display:block;"><br /><u>Résults transformation biobrick R0040/C0040/R0011/C0012</u><br />
Positif for all<br />
</p><p style="display:block;"><br /><u>Minipreps</u><br />
pBM43<br />
From 10 ml of overnight culture to 60μL Vf.<br />
</p><p style="display:block;"><br /><u>electrophoresis Minipreps</u><br />
5μL of each in wells.<br />
</p><p style="display:block;">3μL of 1kb Ladder<br />
</p><p style="display:block;">picture of the gel<br />
</p><p style="display:block;"><br /><br />
<br /><u>Overnight culture </u><br />
(biobrick R0040/C0040/R0011/C0012 in LB + Amp)*2 (one for glycerol)<br />
</p><br />
<ul><li>P1003 LB+Amp+Kan<br />
</li><li>YejF KO (from KEIO collection , KanR disrupt YejF gene) (LB+ Kan)*2 (one for glycerol)<br />
</li></ul><br />
</td></tr><br />
<tr><br />
<td style="background-color: #EEE"><a href="/wiki/Image:Igem-logo-150px.png" class="image" title="Igem-logo-150px.png"><img alt="" src="/images/7/7b/Igem-logo-150px.png" width="150" height="115" border="0" /></a><span style="font-size:22px;">Population counter</span><br />
</td><td style="background-color: #F2F2F2" align="center"><img src="/images/9/94/Report.png" border="0" /> <a href="/wiki/IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010" title="IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010">Main project page</a><br /><img src="/images/c/c3/Resultset_previous.png" border="0" /><a href="/wiki/IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010/2010/07/21" title="IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/21">Previous entry</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/wiki/IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010/2010/07/23" title="IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/23">Next entry</a><img src="/images/5/5c/Resultset_next.png" border="0" /><br />
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<tr><br />
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<p style="display:block;"><br /><font color="red">Aleksandra, Stéphane, Raphaël</font><br />
<br /><u>Minipreps</u>&nbsp;: 60 minipreps in total, 4 minipreps of a 5ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL:<br />
</p><br />
<ul><li> I732006 (LacZ)<br />
</li><li> B0031 (weak RBS)<br />
</li><li> B0032 (medium RBS)<br />
</li><li> B0034 (standard RBS)<br />
</li><li> J31007 (TetA C)<br />
</li><li> C0062 (LuxR w/o RBS)<br />
</li><li> J37033 (RBS/LuxR) from the 2007 distribution plate<br />
</li><li> 23119 (Ps)<br />
</li><li> J23110 (Pm)<br />
</li><li> attC<br />
</li><li> term/attC/mRFP<br />
</li><li> B0015 (term)<br />
</li><li> E0240 (RBS/GFP/term)<br />
</li><li> K081008 (LuxI)<br />
</li><li> R0062 (pLux) <br />
</li></ul><br />
<p style="display:block;"><br /><font color="red">Aleksandra, Raphaël, Théotime</font><br />
<br /><u>Nanodrop</u> to determine the amount of DNA in the miniprep samples:<br />
</p><br />
<table><br />
<tr><br />
<td>Sample name </td><td> 260/280 </td><td>260/230 </td><td>ng/μL<br />
</td></tr><br />
<tr><br />
<td> term (B0015)</td><td>1.82 </td><td>2.34 </td><td>119.9<br />
</td></tr><br />
<tr><br />
<td> attC</td><td>1.72 </td><td>2.21 </td><td>26.8<br />
</td></tr><br />
<tr><br />
<td> RBS/LuxI (K081008) </td><td>1.85 </td><td>2.35 </td><td>102.3<br />
</td></tr><br />
<tr><br />
<td> Pmed (J23110)</td><td> 1.87</td><td>2.34 </td><td>194.2<br />
</td></tr><br />
<tr><br />
<td> Pstr (J23119) </td><td>1.86 </td><td>2.31 </td><td>169.5<br />
</td></tr><br />
<tr><br />
<td> LuxR (C0062) </td><td> 1.86</td><td>2.41 </td><td>188.0<br />
</td></tr><br />
<tr><br />
<td> TetA(C) (J31007)</td><td> 1.86</td><td> 2.35</td><td>149.1<br />
</td></tr><br />
<tr><br />
<td> RBS/LuxR (J37033)</td><td>1.81 </td><td>2.28 </td><td>114.4<br />
</td></tr><br />
<tr><br />
<td> stand.RBS (B0034)</td><td>1.86 </td><td>2.30 </td><td>145.2<br />
</td></tr><br />
<tr><br />
<td> med.RBS (B0032)</td><td>1.90 </td><td>2.34 </td><td>120.1<br />
</td></tr><br />
<tr><br />
<td> weakRBS (B0031)</td><td> 1.86</td><td>2.32 </td><td>163.2<br />
</td></tr><br />
<tr><br />
<td> LacZalpha (I732006) </td><td>1.86 </td><td>2.42 </td><td>221.3<br />
</td></tr><br />
<tr><br />
<td>pLux (R0062) </td><td>1.93 </td><td>2.27 </td><td>66.6<br />
</td></tr><br />
<tr><br />
<td> t/attC/mRFP </td><td>1.97 </td><td>2.13 </td><td>33.1<br />
</td></tr><br />
<tr><br />
<td>RBS/GFP/term (E0240)</td><td>1.89</td><td>2.39</td><td>120.6<br />
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