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Antoine
Overnight culture E.coli TG1 carrying pBM43 (mccABCDE+AmpR+CmR. 2 plates with two different kind of colonies both (big and small). Overnigth culture LB+Cm+Amp for:

• one big colony plate 1
• one small colony plate 1
• one big colony plate 2
• one small colony plate 2

Transformation Biobrick in TOP10

• PlTetO BBa_R0040
• TetR BBa_C0040
• PLacI BBa_R0011
• LacI BBa_C0012

All on Amp plate

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Théotime and Raphaël
Minipreps
2 minipreps for each biobrick from 5ml culture each. Elution of each miniprep with 50µl of Elution Buffer, which gives about 90µl of miniprep per biobrick.

• J732006 (LacZ) -exception : only 3 ml of culture left to work with
• B0031 (weak RBS)
• B0032 (medium RBS)
• B0034 (standard RBS)
• J31007 (TetA C)
• C0062 (LuxR w/o RBS)
• J37033 (RBS/LuxR) from the 2007 distribution plate
• 23119 (Ps) - Amp
• J23110 (Pm) - Amp
• attC -Kan
• term/attC/mRFP -Kan
• B0015 (term) - Amp
• E0240 (RBS/GFP/term) - Amp
• K081008 (LuxI) - Amp
• R0062 (pLux) - Amp

Nanodrop

Glycerol cultures

1125µl culture + 375µl glycerol (15%)

• J732006 (LacZ)
• B0031 (weak RBS)
• B0032 (medium RBS)
• B0034 (standard RBS)
• J31007 (TetA C)
• C0062 (LuxR w/o RBS)
• J37033 (RBS/LuxR) from the 2007 distribution plate

Aleksandra
Transformation of TOP10 cells with 2µl of the ligation product (ligation overnight 07/21-07/22) :

• pLux-RBS/GFP/term - Amp
• attc-term/attC/mRFP -Kan
• LuxI-term -Amp

→The attc-term/attC/mRFP and the LuxI-term plates have colonies! (07/23) Maybe our transformations worked.

Overnight cultures

2 falcons of 10 ml culture each for these parts :

• J732006 (LacZ)
• B0031 (weak RBS)
• B0032 (medium RBS)
• B0034 (standard RBS)
• J31007 (TetA C)
• C0062 (LuxR w/o RBS)
• J37033 (RBS/LuxR) from the 2007 distribution plate
• 23119 (Ps)
• J23110 (Pm)
• attC
• term/attC/mRFP
• B0015 (term)
• E0240 (RBS/GFP/term)
• K081008 (LuxI)
• R0062 (pLux)