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Team:Panama/5 August 2010
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(→Plasmid DNA concentration measurements) |
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==='''August 5'''=== | ==='''August 5'''=== | ||
| - | + | '''Plasmid DNA concentration measurements''' | |
1.'''Promoter:''' 27,8 ng/ul | 1.'''Promoter:''' 27,8 ng/ul | ||
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3.'''Reporter''': 40,3 ng/ul | 3.'''Reporter''': 40,3 ng/ul | ||
| - | ''Digestion mix Promoter and RBS'': | + | '''Digestion mix Promoter and RBS''': |
| - | After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids | + | |
| + | After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids. | ||
[[Image:Cuadro_5agosto.jpg|300px|thumb|left|alt text]] | [[Image:Cuadro_5agosto.jpg|300px|thumb|left|alt text]] | ||
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| - | '''NOTE:''' The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form). | + | '''NOTE:''' The plasmid was not digested because we didn't resuspend it in water (it comes in a lyophilized form). |
'''Electrophoresis of the terminator BioBrick'''. | '''Electrophoresis of the terminator BioBrick'''. | ||
Latest revision as of 03:11, 28 October 2010
August 5
Plasmid DNA concentration measurements
1.Promoter: 27,8 ng/ul
2.RBS: 42 ng/ul
3.Reporter: 40,3 ng/ul
Digestion mix Promoter and RBS:
After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids.
This reaction was incubated at 37ºC for 3 hours, in the thermocycler.
NOTE: The plasmid was not digested because we didn't resuspend it in water (it comes in a lyophilized form).
Electrophoresis of the terminator BioBrick.
We prepared 1% of agarose. In each well we add: 5ul of sample, 2 ul of loading buffer. The marker used is HindIII, 1ul + 2ul loading buffer.
Now, for some reason, the sample didn't stay inside the wells.
[[Image:|350px|thumb|left|alt text]]
The expected band was 2160pb but actually it resulted much higher, aproximately in the 3000pb range and seems too high. This isn´t a satisfactory result because it doesn't have the necessary length.
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