Team:Panama/5 August 2010
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''Digestion mix Promoter and RBS'': | ''Digestion mix Promoter and RBS'': | ||
+ | After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids | ||
[[Image:Cuadro_5agosto.jpg|300px|thumb|left|alt text]] | [[Image:Cuadro_5agosto.jpg|300px|thumb|left|alt text]] | ||
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This reaction was incubated at 37ºC for 3 hours, in the thermocycler. | This reaction was incubated at 37ºC for 3 hours, in the thermocycler. | ||
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Revision as of 04:46, 27 October 2010
August 5
Plasmid DNA concentration measurements
1.Promoter: 27,8 ng/ul
2.RBS: 42 ng/ul
3.Reporter: 40,3 ng/ul
Digestion mix Promoter and RBS: After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids
This reaction was incubated at 37ºC for 3 hours, in the thermocycler.
NOTE: The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form).
Electrophoresis of the terminator BioBrick.
We prepared 1% of agarose. In each well we add: 5ul of sample, 2 ul of loading buffer. The marker used is HindIII, 1ul + 2ul loading buffer.
Now, for some reason, the sample didn't stay inside the wells.
[[Image:|350px|thumb|left|alt text]]
The expected band was 2160pb but actually it resulted much higher, aproximately in the 3000pb range and seems too high. This isn´t a satisfactory result because it doesn't have the necessary length.
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