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Team:Panama/4 October 2010
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PCR (About 2.5 hours) | PCR (About 2.5 hours) | ||
| - | On October 1, we did the miniprep of the cloning product of the gene | + | On October 1, we did the miniprep of the cloning product of the gene Rh1AB in the pGEM-T vector |
Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1AB | Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1AB | ||
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| - | No bands appear | + | No bands appear. =( |
Possible reasons: | Possible reasons: | ||
| - | 1) The PCR has had some sort of problem | + | 1) The PCR has had some sort of problem. |
| - | 2) The cloning has had some sort of problem | + | 2) The cloning has had some sort of problem. |
Possible solutions: | Possible solutions: | ||
| - | 1) Repeat the PCR, changing the denaturing temperature time to 8 minutes and in the cycling from 30 seconds to 1 minute. Besides that, we used two different miniprep samples from the cloning (maybe the colony that we picked doesn't have the insert) | + | 1) Repeat the PCR, changing the denaturing temperature time to 8 minutes and in the cycling from 30 seconds to 1 minute. Besides that, we used two different miniprep samples from the cloning (maybe the colony that we picked doesn't have the insert). |
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Latest revision as of 00:57, 28 October 2010
October 4
Ligation Reaction
Ligation of Reporter and Terminator (About two hours)
We used the same quantities that were used on September 23 2010
PCR (About 2.5 hours)
On October 1, we did the miniprep of the cloning product of the gene Rh1AB in the pGEM-T vector
Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1AB
No bands appear. =(
Possible reasons:
1) The PCR has had some sort of problem.
2) The cloning has had some sort of problem.
Possible solutions:
1) Repeat the PCR, changing the denaturing temperature time to 8 minutes and in the cycling from 30 seconds to 1 minute. Besides that, we used two different miniprep samples from the cloning (maybe the colony that we picked doesn't have the insert).
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