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Team:Panama/27 July 2010
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Revision as of 20:31, 11 October 2010 by Ezequiel.aguilar (Talk | contribs)
July 27
Miniprep Today we performed the miniprep of the E. coli transformed with the RBS and Promoter parts. This miniprep was made in triplicate.
Electrophoresis After the miniprep we made an electrophoresis in 1% agarose gel. Also, we measured the DNA concentration with a spectrophotometer:
[RBS]=30,4 ηg/μl
Digestion After the DNA concentration measurement, we performed the digestion of the RBS and Promoter parts.
Digestion mix for the Promoter:
NEBuffer2=5μl
BSA=0,5 μl
EcoRI=1 μl
SpeI=1 μl
DNA=20 μl
H2O=22,5μl
Digestion mix for the RBS:
NEBuffer2=5μl
BSA=0,5 μl
XbaI=1 μl
PstI=1 μl
DNA=20 μl
H2O=22,5μl
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