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Team:Panama/21 October 2010
From 2010.igem.org
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==='''October 21'''=== | ==='''October 21'''=== | ||
Revision as of 18:11, 23 October 2010
October 21
Electrophoresis: Miniprep Samples 0.8% agarose, 1x TBE, 1.5 ul EtBr
- Photo
Sample 6 is a possible plasmid with insert
1) PCR sample 6
Master Mix 12.5 ul
Primer F 1 ul
Primer R 1 ul
DNA 2 ul
Water 8.5 ul
Total 25 ul
2. Digest with EcoR1
Digestion of Sample 6 with EcoR1 Take 1.21 ul for 100 ng
Calculations for Mutagenesis Primers
1. 300 ug/ 334 ul water = 898 ng/ul
1/10 dilution
Take 1.11 ul of diluted stock for PCR
2. 230 ug/279 ul water we have this concentration of primers diluted which is the same as 824 ng/ul Do 1/10 dilution (90 ul water and 10 ul of oligo Take 1.21 ul of diluted stock for PCR
3. 280 ug/446 ul water = 600 ng/ul Dilute 1/10 Take 1.67 ul of diluted stock for PCR
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