Team:Panama/20 October 2010
From 2010.igem.org
(Difference between revisions)
(→October 20) |
(→October 20) |
||
Line 3: | Line 3: | ||
[[Image:Cuadro20octubre1.JPG|200px|thumb|left|alt text]] | [[Image:Cuadro20octubre1.JPG|200px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
Line 8: | Line 12: | ||
[[Image:UntitledGel20Octubre1.JPG|200px|thumb|left|alt text]] | [[Image:UntitledGel20Octubre1.JPG|200px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
Line 40: | Line 50: | ||
[[Image:Cuadro20octubre2.JPG|400px|thumb|left|alt text]] | [[Image:Cuadro20octubre2.JPG|400px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
Line 50: | Line 64: | ||
[[Image:UntitledGel20Octubre2.JPG|400px|thumb|left|alt text]] | [[Image:UntitledGel20Octubre2.JPG|400px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
Line 57: | Line 80: | ||
[[Image:Cuadro20octubre3.JPG|400px|thumb|left|alt text]] | [[Image:Cuadro20octubre3.JPG|400px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
Revision as of 00:05, 28 October 2010
October 20
Digestion of pGEM-T + Rh1ab to compare with the non-digested and verify the difference in size Note: EcoR1 releases the insert.
Quick Check: Transformation of E. Coli with pGEM-T +Rh1ab
-100 ul culture -50 ul phenol:chloroform -10 ul loading buffer
Vortex
Electrophoresis: Quick Check 0.8% agarose, with 1x TBE, 1.5 ul EtBr Samples:4, 14, 13, 22, 8, 6, 16, 12, 9, 25, 5, 1 (C-), 15, 27, 28, 10, 3, 31, 29, 11
Homemade minipreps of Samples 1, 3, 5, 6, 10, 11, 12, 13, 16, 31
Cloning: pGEM-T + Rh1ab -Ligation: Overnight at 16C Ratio of insert:vector as 1:1
3 ul of PCR product
|
|
|
|
|
|