Team:Panama/20 October 2010

From 2010.igem.org

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Revision as of 00:05, 28 October 2010

iGEM Panama

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October 20

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Digestion of pGEM-T + Rh1ab to compare with the non-digested and verify the difference in size Note: EcoR1 releases the insert.

Quick Check: Transformation of E. Coli with pGEM-T +Rh1ab

-100 ul culture -50 ul phenol:chloroform -10 ul loading buffer

Vortex

Electrophoresis: Quick Check 0.8% agarose, with 1x TBE, 1.5 ul EtBr Samples:4, 14, 13, 22, 8, 6, 16, 12, 9, 25, 5, 1 (C-), 15, 27, 28, 10, 3, 31, 29, 11


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Homemade minipreps of Samples 1, 3, 5, 6, 10, 11, 12, 13, 16, 31

Cloning: pGEM-T + Rh1ab -Ligation: Overnight at 16C Ratio of insert:vector as 1:1

3 ul of PCR product


May
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31