Team:Panama/20 August 2010
From 2010.igem.org
(Difference between revisions)
(→August 20) |
(→August 20) |
||
Line 57: | Line 57: | ||
- | [[Image:20-8.JPG|300px|thumb| | + | [[Image:20-8.JPG|300px|thumb|medial|alt text]] |
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} |
Revision as of 01:00, 26 October 2010
August 20
Restriction reactions
Today the work was focused on the digestion reaction of three parts, RBS, Promotor and the plasmid.
2) RBS
Buffer 2 ------------> 0,5 ul
BSA -----------------> 5 ul
Xba -----------------> 1 ul
Pst1 ----------------> 1 ul
DNA(BBa_B0034)-------> 20 ul
H2O -----------------> 22,5 ul
Final volume= 50 ul.
3) Plasmid
Buffer 2 ------------> 0,5 ul
BSA -----------------> 5 ul
EcoR1----------------> 1 ul
Spel ----------------> 1 ul
DNA(BBa_psB1A2)------> 20 ul
H2O -----------------> 22,5 ul
Final volume= 50 ul.
For the sample visualization, we performed an electrophosis on a agarose gel.
|
|
|
|
|
|