Team:Panama/13 August 2010

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'''Digestion Reaction'''
'''Digestion Reaction'''
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Today we did the digest the plasmid that contain the promoter with the EcoRI and SpeI enzymes, and the plasmid with the RBS was digested with the XhaI and PstI enzymes.
[[Image:Cuadro_13_agosto.jpg|300px|thumb|left|alt text]]
[[Image:Cuadro_13_agosto.jpg|300px|thumb|left|alt text]]
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== '''PCR Rhlab amplification''' ==
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Revision as of 05:14, 27 October 2010

iGEM Panama

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August 13

Digestion Reaction Today we did the digest the plasmid that contain the promoter with the EcoRI and SpeI enzymes, and the plasmid with the RBS was digested with the XhaI and PstI enzymes.

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RESULTS

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PCR Rhlab amplification

Now that we have our Rh1ab we are going to run a PCR of it using our first set of primers.

RhT-F1a 5' GCGATAGCTGTTTGCCTGTT 3'

RhT-R2a 5' TGGCAACCCTATCTGTTATGC 3'

First of all, we have to resuspend our primers since they come in a lyophilized manner. We need a stock solution of 100uM. Our working solution has a concentration of 10uM.

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RESULTS

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We tested three concentrations of primers to see which one worked better for us and we can see that all three concentrations worked! So from now on, just to be on the safe side, we're going to use the concentration of 1uM.

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