Team:Panama/12 August 2010

From 2010.igem.org

iGEM Panama

  • Decrease font size
  • Default font size
  • Increase font size
  • default color
  • color1 color
  • color2 color
  • color3 color

August 12

And more minipreps

Today we performed the plasmidic DNA extraction from the RBS and promoter using a Promega Kit.

DNA concentration measurement

RBS 1 = 6.5ng/ul

RBS 2 = 9.5ng/ul

Promoter 1 = 12.2ng/ul

Promoter 2 = 14.1ng/ul

P. aeruginosa 1 = 6.0ng/ul

P. aeruginosa 2 = 98ng/ul

P. aeruginosa 3 = 75ng/ul


DNA Precipitation:

1. We placed twice the volume of absolute ethanol for 15 minutes in ice. To that we added 12.5ul of NaC2H3O2 2M.

2. This was centrifuged at 12000RPM for 5 minutes.

3. We washed with twice the volume of ethanol at 70%.

4. Again this was centrifuged at the previous conditions.

5. The supernatant was eliminated.

6. After this, we let the ethanol dry up.

7. The product was resuspended in 20ul of DNAse free water.

May
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31