Team:Osaka/Test

From 2010.igem.org

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{{Osaka}}
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{{Osaka_countdown}} till iGEM Jamboree 2010
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<h1 style="text-align: left">NOTES</h1>
 
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<span class="bold">CONTINUOUS GREENING CYCLE</span><br>
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Desertification all over the world causes famine, drought and suffering. We aim to develop micro-machines that can stop and even reverse desertification by recovering vegetation in these areas. We envison a <i>Continuous Greening Cycle</i> in which engineered microorganisms decompose plant fibers into nutrients through the action of cellulolytic enzymes. They then produce water-holding polymers such as poly(gamma-glutamic) acid that retain water in the soil to help plants grow. When the plants die they will be decomposed to start the cycle anew. In addition to aiming for the continuous and self-expanding greening of desert areas, we hope to contribute to iGEM by developing useful BioBricks!<br>
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<img src="https://static.igem.org/mediawiki/2010/d/df/Continuous_Greening_Cycle.jpg" alt="Continuous Greening Cycle" height="380" width="520">
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{{Osaka_twitter}}
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  <th valign=top>{{#calendar: title=July |year=2009 | month=07}}</th>
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  <th valign=top>{{#calendar: title=September |year=2009 | month=09}}</th>
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    <h2>Registry Parts</h2></div>
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<h3>All of Osaka's Parts and Devices is <a href="https://2009.igem.org/Team:Osaka/Parts_%26_Devices">here</a></h3>
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<h4>COLOR</h4>
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<p><a href="http://partsregistry.org/Part:BBa_K204033">K204033</a> mOrange</p>
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placI
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<p><a href="http://partsregistry.org/Part:BBa_K204041">K204041</a> placI+GFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204042">K204042</a> placI+YFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204043">K204043</a> placI+CFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204044">K204044</a> placI+RFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204048">K204048</a> placI+mOrange</p>
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pcI
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<p><a href="http://partsregistry.org/Part:BBa_K204058">K204058</a> pcI+YFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204059">K204059</a> pcI+CFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204062">K204062</a> pcI+mOrange</p>
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ptetR
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<p><a href="http://partsregistry.org/Part:BBa_K204052">K204052</a> ptetR+mOrange</p>
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Carotenoid
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<p><a href="http://partsregistry.org/Part:BBa_K204064">K204064</a> crtE+crtB<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204065">K204065</a> crtI+crtY<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204068">K204068</a> crtZ<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204076">K204076</a> placI+crtE+crtB</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204077">K204077</a> ptetR+crtE+crtB</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204091">K204091</a> placI+crtE+crtB+crtI+crtY</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204095">K204095</a> placI+crtE+crtB+crtZ</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204096">K204096</a> ptetR+crtE+crtB+crtZ</p>
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gradation
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<p><a href="http://partsregistry.org/Part:BBa_K204069">K204069</a> cI repressor</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204086">K204086</a> 3O<sub>6</sub>HSL receiver+RFP+cI repressor</p>
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<h4>SIGNAL </b> (Quorum Sensing)</h4>
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AI-1
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<p><a href="http://partsregistry.org/Part:BBa_K204047">K204047</a> LasI generater</p>
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C<sub>4</sub>HSL
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<p><a href="http://partsregistry.org/Part:BBa_K204040">K204040</a> RhlI (generater)<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204031">K204031</a> RhlR (receiver)<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204702">K204702</a> RhlR+GFP<br></p>
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3OH,C<sub>14</sub> : 1-HSL
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<p><a href="http://partsregistry.org/Part:BBa_K204025">K204025</a> cinI (generater)</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204032">K204032</a> cinR (receiver)</p>
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3O<sub>6</sub>HSL
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<p><a href="http://partsregistry.org/Part:BBa_K204051">K204051</a> LuxR (receiver)</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204700">K204700</a> LuxR+GFP</p>
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<h4>MOTILITY</h4>
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<p><a href="http://partsregistry.org/Part:BBa_K204018">K204018</a> ptetR+EspE</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204501">K204501</a> RBS+FliH</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204502">K204502</a> ptetR+FliH</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204503">K204503</a> pT7+FliH</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204504">K204504</a> C<sub>4</sub>HSL receiver +FliH</p>
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  <h3>Wet Lab</h3>
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<p>Week 1 : 8/2~8/8</p>
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<p>Brainstorming , parts planning , prepared competent cell</p>
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<p>ransformation and miniprep from MIT plate</p>
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<p>week 2 : 8/9~8/15</p>
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<p>start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT</p>
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<p>Week 3 : 8/16~8/22</p>
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<p>assembly parts (~K204015)  ,  parts planning</p>
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<p>Week 4 : 8/23~8/29</p>
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<p>assembly parts (~K204021)</p>
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<p>Week 5 : 8/30~9/5</p>
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<p>assembly parts (~K204030)</p>
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<p>Week 6 : 9/6~9/12</p>
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<p>assembly parts (~K204046)</p>
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<p>Week 7 : 9/13~9/19</p>
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<p>assembly parts (~K204050)</p>
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<p>Week 8 : 9/20~9/26</p>
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<p>assembly parts (~K204056)</p>
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<p>TEST : sensitivity of inducer and receptor</p>
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<p>Sample : 3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL</p>
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<p>Result : Not confirm the fluorescence...</p>
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<p>Week 9 : 9/27~10/3</p>
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<p>assembly parts (~K204066 , X1~X8), color (fluorescence) intensity check</p>
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<p>Week 10 : 10/4~10/10</p>
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<p>assembly parts (~K204078)</p>
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<p>Lux receiver sensitivity test, color (fluorescence) intensity check</p>
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<p>sequence check : generator and receiver</p>
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<p>3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL</p>
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<h4 style="text-indent:2em">Get the parts from Team Chiba. Thanks a lot!!</h4>
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<p>Week 11 : 10/11~10/17</p>
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<p>assembly parts (~K2040104)</p>
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<p>sequence check : FliH etc…</p>
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<p>send Osaka team parts to MIT</p>
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<p>FINISH!</p>
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<h4 style="text-indent:2em">Finally we assembled about 120 parts!!</h4>
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<h3>Dry Lab</h3>
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<p>Week 5 : 8/30~9/5</p>
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<p>Planing model.</p>
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<p>Week 6 : 9/6~9/12</p>
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<p>Studying programing using Matlab. </p>
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<p>Week 7 : 9/13~9/19</p>
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<p>Making program in case of single coloney.</p>
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<p>Week 8 : 9/20~9/26</p>
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<p>Making program in case of more than two colonies</p>
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<p>Making movie file</p>
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<p>Searching paper written some parameter</p>
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<p>Week 9 : 9/27~10/3</p>
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<p>Blash up our program </p>
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<p>Searching paper written some parameter</p>
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<p>Week 10 : 10/4~10/10</p>
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<p>Making program of color gradation</p>
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<p>Week 11 : 10/11~10/17</p>
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<p>Blash up our program</p>
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<h3>Atelie</h3>
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<p>Week 8 : 9/20~9/26</p>
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<p>Making art works</p>
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<p>Week 9 : 9/27~10/3</p>
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<p>Making art works</p>
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<p>Week 10 : 10/4~10/10</p>
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<p>Making art works</p>
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<p>Week 11 : 10/11~10/17</p>
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<p>Making art works</p>
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Latest revision as of 02:14, 2 October 2010

till iGEM Jamboree 2010
CONTINUOUS GREENING CYCLE
Desertification all over the world causes famine, drought and suffering. We aim to develop micro-machines that can stop and even reverse desertification by recovering vegetation in these areas. We envison a Continuous Greening Cycle in which engineered microorganisms decompose plant fibers into nutrients through the action of cellulolytic enzymes. They then produce water-holding polymers such as poly(gamma-glutamic) acid that retain water in the soil to help plants grow. When the plants die they will be decomposed to start the cycle anew. In addition to aiming for the continuous and self-expanding greening of desert areas, we hope to contribute to iGEM by developing useful BioBricks!
Continuous Greening Cycle

Retrieved from "http://2010.igem.org/Team:Osaka/Test"