Team:Osaka/Test

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<title>Fuck yeah!</title>
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    <h2>Registry Parts</h2></div>
 
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<h3>All of Osaka's Parts and Devices is <a href="https://2009.igem.org/Team:Osaka/Parts_%26_Devices">here</a></h3>
 
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<h4>COLOR</h4>
 
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<br>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204033">K204033</a> mOrange</p>
 
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placI
 
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<p><a href="http://partsregistry.org/Part:BBa_K204041">K204041</a> placI+GFP</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204042">K204042</a> placI+YFP</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204043">K204043</a> placI+CFP</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204044">K204044</a> placI+RFP</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204048">K204048</a> placI+mOrange</p>
 
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pcI
 
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<p><a href="http://partsregistry.org/Part:BBa_K204058">K204058</a> pcI+YFP</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204059">K204059</a> pcI+CFP</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204062">K204062</a> pcI+mOrange</p>
 
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ptetR
 
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<p><a href="http://partsregistry.org/Part:BBa_K204052">K204052</a> ptetR+mOrange</p>
 
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Carotenoid
 
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<p><a href="http://partsregistry.org/Part:BBa_K204064">K204064</a> crtE+crtB<br></p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204065">K204065</a> crtI+crtY<br></p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204068">K204068</a> crtZ<br></p>
 
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<br>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204076">K204076</a> placI+crtE+crtB</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204077">K204077</a> ptetR+crtE+crtB</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204091">K204091</a> placI+crtE+crtB+crtI+crtY</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204095">K204095</a> placI+crtE+crtB+crtZ</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204096">K204096</a> ptetR+crtE+crtB+crtZ</p>
 
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gradation
 
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<p><a href="http://partsregistry.org/Part:BBa_K204069">K204069</a> cI repressor</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204086">K204086</a> 3O<sub>6</sub>HSL receiver+RFP+cI repressor</p>
 
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<br>
 
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<h4>SIGNAL </b> (Quorum Sensing)</h4>
 
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AI-1
 
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<p><a href="http://partsregistry.org/Part:BBa_K204047">K204047</a> LasI generater</p>
 
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C<sub>4</sub>HSL
 
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<p><a href="http://partsregistry.org/Part:BBa_K204040">K204040</a> RhlI (generater)<br></p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204031">K204031</a> RhlR (receiver)<br></p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204702">K204702</a> RhlR+GFP<br></p>
 
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3OH,C<sub>14</sub> : 1-HSL
 
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<p><a href="http://partsregistry.org/Part:BBa_K204025">K204025</a> cinI (generater)</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204032">K204032</a> cinR (receiver)</p>
 
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3O<sub>6</sub>HSL
 
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<p><a href="http://partsregistry.org/Part:BBa_K204051">K204051</a> LuxR (receiver)</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204700">K204700</a> LuxR+GFP</p>
 
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<h4>MOTILITY</h4>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204018">K204018</a> ptetR+EspE</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204501">K204501</a> RBS+FliH</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204502">K204502</a> ptetR+FliH</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204503">K204503</a> pT7+FliH</p>
 
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<p><a href="http://partsregistry.org/Part:BBa_K204504">K204504</a> C<sub>4</sub>HSL receiver +FliH</p>
 
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    <h2>Lab Note</h2></div>
 
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  <h3>Wet Lab</h3>
 
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<p>Week 1 : 8/2~8/8</p>
 
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<p>Brainstorming , parts planning , prepared competent cell</p>
 
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<p>ransformation and miniprep from MIT plate</p>
 
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<br>
 
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<p>week 2 : 8/9~8/15</p>
 
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<p>start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT</p>
 
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<br>
 
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<p>Week 3 : 8/16~8/22</p>
 
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<p>assembly parts (~K204015)  ,  parts planning</p>
 
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<br>
 
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<p>Week 4 : 8/23~8/29</p>
 
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<p>assembly parts (~K204021)</p>
 
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<br>
 
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<p>Week 5 : 8/30~9/5</p>
 
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<p>assembly parts (~K204030)</p>
 
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<br>
 
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<p>Week 6 : 9/6~9/12</p>
 
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<p>assembly parts (~K204046)</p>
 
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<br>
 
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<p>Week 7 : 9/13~9/19</p>
 
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<p>assembly parts (~K204050)</p>
 
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<br>
 
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<p>Week 8 : 9/20~9/26</p>
 
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<p>assembly parts (~K204056)</p>
 
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<p>TEST : sensitivity of inducer and receptor</p>
 
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<p>Sample : 3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL</p>
 
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<p>Result : Not confirm the fluorescence...</p>
 
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<br>
 
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<p>Week 9 : 9/27~10/3</p>
 
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<p>assembly parts (~K204066 , X1~X8), color (fluorescence) intensity check</p>
 
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<br>
 
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<p>Week 10 : 10/4~10/10</p>
 
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<p>assembly parts (~K204078)</p>
 
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<p>Lux receiver sensitivity test, color (fluorescence) intensity check</p>
 
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<p>sequence check : generator and receiver</p>
 
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<p>3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL</p>
 
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<h4 style="text-indent:2em">Get the parts from Team Chiba. Thanks a lot!!</h4>
 
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<br>
 
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<p>Week 11 : 10/11~10/17</p>
 
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<p>assembly parts (~K2040104)</p>
 
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<p>sequence check : FliH etc…</p>
 
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<p>send Osaka team parts to MIT</p>
 
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<p>FINISH!</p>
 
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<h4 style="text-indent:2em">Finally we assembled about 120 parts!!</h4>
 
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<h3>Dry Lab</h3>
 
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<p>Week 5 : 8/30~9/5</p>
 
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<p>Planing model.</p>
 
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<br>
 
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<p>Week 6 : 9/6~9/12</p>
 
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<p>Studying programing using Matlab. </p>
 
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<br>
 
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<p>Week 7 : 9/13~9/19</p>
 
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<p>Making program in case of single coloney.</p>
 
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<br>
 
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<p>Week 8 : 9/20~9/26</p>
 
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<p>Making program in case of more than two colonies</p>
 
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<p>Making movie file</p>
 
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<p>Searching paper written some parameter</p>
 
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<br>
 
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<p>Week 9 : 9/27~10/3</p>
 
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<p>Blash up our program </p>
 
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<p>Searching paper written some parameter</p>
 
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<br>
 
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<p>Week 10 : 10/4~10/10</p>
 
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<p>Making program of color gradation</p>
 
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<br>
 
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<p>Week 11 : 10/11~10/17</p>
 
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<p>Blash up our program</p>
 
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<br>
 
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<h3>Atelie</h3>
 
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<p>Week 8 : 9/20~9/26</p>
 
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<p>Making art works</p>
 
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<br>
 
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<p>Week 9 : 9/27~10/3</p>
 
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<p>Making art works</p>
 
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<br>
 
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<p>Week 10 : 10/4~10/10</p>
 
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<p>Making art works</p>
 
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<br>
 
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<p>Week 11 : 10/11~10/17</p>
 
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<p>Making art works</p>
 
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Revision as of 03:13, 29 August 2010

Fuck yeah!

Test Panel
blablablablabla

Retrieved from "http://2010.igem.org/Team:Osaka/Test"