Team:Osaka/Test

From 2010.igem.org

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bbpart <bbpart>K204031</bbpart>
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  <th valign=top>{{#calendar: title=July |year=2009 | month=07}}</th>
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<count_promoters> <groupparts>iGEM009 Osaka</groupparts></count_promoters>
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partinfo <partinfo>K204031 SequenceAndFeatures</partinfo>
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  <th valign=top>{{#calendar: title=August |year=2009 | month=10}}</th>
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parttable <parttable>iGEM009 Osaka</parttable>
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parttable2 <parttable2>iGEM009 Osaka</parttable2>
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  <div id="CollapsiblePanel2" class="CollapsiblePanel">
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  <div class="CollapsiblePanelTab" tabindex="0">
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    <h2>Registry Parts</h2></div>
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  <div class="CollapsiblePanelContent">
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<h3>All of Osaka's Parts and Devices is <a href="https://2009.igem.org/Team:Osaka/Parts_%26_Devices">here</a></h3>
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<h4>COLOR</h4>
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<br>
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<p><a href="http://partsregistry.org/Part:BBa_K204033">K204033</a> mOrange</p>
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placI
 +
<p><a href="http://partsregistry.org/Part:BBa_K204041">K204041</a> placI+GFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204042">K204042</a> placI+YFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204043">K204043</a> placI+CFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204044">K204044</a> placI+RFP</p>
 +
<p><a href="http://partsregistry.org/Part:BBa_K204048">K204048</a> placI+mOrange</p>
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pcI
 +
<p><a href="http://partsregistry.org/Part:BBa_K204058">K204058</a> pcI+YFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204059">K204059</a> pcI+CFP</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204062">K204062</a> pcI+mOrange</p>
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ptetR
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<p><a href="http://partsregistry.org/Part:BBa_K204052">K204052</a> ptetR+mOrange</p>
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Carotenoid
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<p><a href="http://partsregistry.org/Part:BBa_K204064">K204064</a> crtE+crtB<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204065">K204065</a> crtI+crtY<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204068">K204068</a> crtZ<br></p>
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<br>
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<p><a href="http://partsregistry.org/Part:BBa_K204076">K204076</a> placI+crtE+crtB</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204077">K204077</a> ptetR+crtE+crtB</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204091">K204091</a> placI+crtE+crtB+crtI+crtY</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204095">K204095</a> placI+crtE+crtB+crtZ</p>
 +
<p><a href="http://partsregistry.org/Part:BBa_K204096">K204096</a> ptetR+crtE+crtB+crtZ</p>
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gradation
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<p><a href="http://partsregistry.org/Part:BBa_K204069">K204069</a> cI repressor</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204086">K204086</a> 3O<sub>6</sub>HSL receiver+RFP+cI repressor</p>
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<br>
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<h4>SIGNAL </b> (Quorum Sensing)</h4>
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AI-1
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<legend>Personalia:</legend>
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<p><a href="http://partsregistry.org/Part:BBa_K204047">K204047</a> LasI generater</p>
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Name: <input type="text" size="30" /><br />
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C<sub>4</sub>HSL
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Email: <input type="text" size="30" /><br />
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<p><a href="http://partsregistry.org/Part:BBa_K204040">K204040</a> RhlI (generater)<br></p>
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Date of birth: <input type="text" size="10" />
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<p><a href="http://partsregistry.org/Part:BBa_K204031">K204031</a> RhlR (receiver)<br></p>
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<p><a href="http://partsregistry.org/Part:BBa_K204702">K204702</a> RhlR+GFP<br></p>
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</form>
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3OH,C<sub>14</sub> : 1-HSL
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<p><a href="http://partsregistry.org/Part:BBa_K204025">K204025</a> cinI (generater)</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204032">K204032</a> cinR (receiver)</p>
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3O<sub>6</sub>HSL
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<p><a href="http://partsregistry.org/Part:BBa_K204051">K204051</a> LuxR (receiver)</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204700">K204700</a> LuxR+GFP</p>
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<br>
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<h4>MOTILITY</h4>
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<p><a href="http://partsregistry.org/Part:BBa_K204018">K204018</a> ptetR+EspE</p>
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<br>
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<p><a href="http://partsregistry.org/Part:BBa_K204501">K204501</a> RBS+FliH</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204502">K204502</a> ptetR+FliH</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204503">K204503</a> pT7+FliH</p>
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<p><a href="http://partsregistry.org/Part:BBa_K204504">K204504</a> C<sub>4</sub>HSL receiver +FliH</p>
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    <h2>Lab Note</h2></div>
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  <div class="CollapsiblePanelContent">
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  <h3>Wet Lab</h3>
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<p>Week 1 : 8/2~8/8</p>
 +
<p>Brainstorming , parts planning , prepared competent cell</p>
 +
<p>ransformation and miniprep from MIT plate</p>
 +
<br>
 +
<p>week 2 : 8/9~8/15</p>
 +
<p>start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT</p>
 +
<br>
 +
<p>Week 3 : 8/16~8/22</p>
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<p>assembly parts (~K204015)  ,  parts planning</p>
 +
<br>
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<p>Week 4 : 8/23~8/29</p>
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<p>assembly parts (~K204021)</p>
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<br>
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<p>Week 5 : 8/30~9/5</p>
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<p>assembly parts (~K204030)</p>
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<br>
 +
<p>Week 6 : 9/6~9/12</p>
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<p>assembly parts (~K204046)</p>
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<br>
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<p>Week 7 : 9/13~9/19</p>
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<p>assembly parts (~K204050)</p>
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<br>
 +
<p>Week 8 : 9/20~9/26</p>
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<p>assembly parts (~K204056)</p>
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<p>TEST : sensitivity of inducer and receptor</p>
 +
<p>Sample : 3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL</p>
 +
<p>Result : Not confirm the fluorescence...</p>
 +
<br>
 +
<p>Week 9 : 9/27~10/3</p>
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<p>assembly parts (~K204066 , X1~X8), color (fluorescence) intensity check</p>
 +
<br>
 +
<p>Week 10 : 10/4~10/10</p>
 +
<p>assembly parts (~K204078)</p>
 +
<p>Lux receiver sensitivity test, color (fluorescence) intensity check</p>
 +
<p>sequence check : generator and receiver</p>
 +
<p>3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL</p>
 +
<h4 style="text-indent:2em">Get the parts from Team Chiba. Thanks a lot!!</h4>
 +
<br>
 +
<p>Week 11 : 10/11~10/17</p>
 +
<p>assembly parts (~K2040104)</p>
 +
<p>sequence check : FliH etc…</p>
 +
<p>send Osaka team parts to MIT</p>
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<p>FINISH!</p>
 +
<h4 style="text-indent:2em">Finally we assembled about 120 parts!!</h4>
 +
<br>
 +
<h3>Dry Lab</h3>
 +
<p>Week 5 : 8/30~9/5</p>
 +
<p>Planing model.</p>
 +
<br>
 +
<p>Week 6 : 9/6~9/12</p>
 +
<p>Studying programing using Matlab. </p>
 +
<br>
 +
<p>Week 7 : 9/13~9/19</p>
 +
<p>Making program in case of single coloney.</p>
 +
<br>
 +
<p>Week 8 : 9/20~9/26</p>
 +
<p>Making program in case of more than two colonies</p>
 +
<p>Making movie file</p>
 +
<p>Searching paper written some parameter</p>
 +
<br>
 +
<p>Week 9 : 9/27~10/3</p>
 +
<p>Blash up our program </p>
 +
<p>Searching paper written some parameter</p>
 +
<br>
 +
<p>Week 10 : 10/4~10/10</p>
 +
<p>Making program of color gradation</p>
 +
<br>
 +
<p>Week 11 : 10/11~10/17</p>
 +
<p>Blash up our program</p>
 +
<br>
 +
<h3>Atelie</h3>
 +
<p>Week 8 : 9/20~9/26</p>
 +
<p>Making art works</p>
 +
<br>
 +
<p>Week 9 : 9/27~10/3</p>
 +
<p>Making art works</p>
 +
<br>
 +
<p>Week 10 : 10/4~10/10</p>
 +
<p>Making art works</p>
 +
<br>
 +
<p>Week 11 : 10/11~10/17</p>
 +
<p>Making art works</p>
 +
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{{Template:NOTES_bottom}}
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__NEWSECTIONLINK__
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Revision as of 02:42, 29 August 2010

Home of iGEMOSAKA wiki

NOTES

Calendar

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30


Registry Parts

All of Osaka's Parts and Devices is here

COLOR


K204033 mOrange

placI

K204041 placI+GFP

K204042 placI+YFP

K204043 placI+CFP

K204044 placI+RFP

K204048 placI+mOrange

pcI

K204058 pcI+YFP

K204059 pcI+CFP

K204062 pcI+mOrange

ptetR

K204052 ptetR+mOrange

Carotenoid

K204064 crtE+crtB

K204065 crtI+crtY

K204068 crtZ


K204076 placI+crtE+crtB

K204077 ptetR+crtE+crtB

K204091 placI+crtE+crtB+crtI+crtY

K204095 placI+crtE+crtB+crtZ

K204096 ptetR+crtE+crtB+crtZ

gradation

K204069 cI repressor

K204086 3O6HSL receiver+RFP+cI repressor


SIGNAL (Quorum Sensing)

AI-1

K204047 LasI generater

C4HSL

K204040 RhlI (generater)

K204031 RhlR (receiver)

K204702 RhlR+GFP

3OH,C14 : 1-HSL

K204025 cinI (generater)

K204032 cinR (receiver)

3O6HSL

K204051 LuxR (receiver)

K204700 LuxR+GFP


MOTILITY

K204018 ptetR+EspE


K204501 RBS+FliH

K204502 ptetR+FliH

K204503 pT7+FliH

K204504 C4HSL receiver +FliH


Lab Note

Wet Lab

Week 1 : 8/2~8/8

Brainstorming , parts planning , prepared competent cell

ransformation and miniprep from MIT plate


week 2 : 8/9~8/15

start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT


Week 3 : 8/16~8/22

assembly parts (~K204015) , parts planning


Week 4 : 8/23~8/29

assembly parts (~K204021)


Week 5 : 8/30~9/5

assembly parts (~K204030)


Week 6 : 9/6~9/12

assembly parts (~K204046)


Week 7 : 9/13~9/19

assembly parts (~K204050)


Week 8 : 9/20~9/26

assembly parts (~K204056)

TEST : sensitivity of inducer and receptor

Sample : 3O6HSL , C4HSL , AI-1 , 3OH,C14:1-HSL

Result : Not confirm the fluorescence...


Week 9 : 9/27~10/3

assembly parts (~K204066 , X1~X8), color (fluorescence) intensity check


Week 10 : 10/4~10/10

assembly parts (~K204078)

Lux receiver sensitivity test, color (fluorescence) intensity check

sequence check : generator and receiver

3O6HSL , C4HSL , AI-1 , 3OH,C14:1-HSL

Get the parts from Team Chiba. Thanks a lot!!


Week 11 : 10/11~10/17

assembly parts (~K2040104)

sequence check : FliH etc…

send Osaka team parts to MIT

FINISH!

Finally we assembled about 120 parts!!


Dry Lab

Week 5 : 8/30~9/5

Planing model.


Week 6 : 9/6~9/12

Studying programing using Matlab.


Week 7 : 9/13~9/19

Making program in case of single coloney.


Week 8 : 9/20~9/26

Making program in case of more than two colonies

Making movie file

Searching paper written some parameter


Week 9 : 9/27~10/3

Blash up our program

Searching paper written some parameter


Week 10 : 10/4~10/10

Making program of color gradation


Week 11 : 10/11~10/17

Blash up our program


Atelie

Week 8 : 9/20~9/26

Making art works


Week 9 : 9/27~10/3

Making art works


Week 10 : 10/4~10/10

Making art works


Week 11 : 10/11~10/17

Making art works


Template:NOTES bottom

Retrieved from "http://2010.igem.org/Team:Osaka/Test"