Team:Osaka/Protocols

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Protocols

  • Preparation of competent cells for heat-shock transformation
  • Transformation by heat-shock
  • Plasmid DNA miniprep
  • 3A BioBrick assembly
  • PCR cloning
  • PCR site-directed mutagenesis
  • Extraction of genome DNA for PCR cloning

Retrieved from "http://2010.igem.org/Team:Osaka/Protocols"