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Team:Osaka/Protocols
From 2010.igem.org
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| - | + | <h2>Protocols</h2> | |
| - | + | <ul> | |
| - | + | <li>Preparation of competent cells for heat-shock transformation | |
| - | + | <li>Transformation by heat-shock | |
| - | + | <li>Plasmid DNA miniprep | |
| - | + | <li>3A BioBrick assembly | |
| - | + | <li>PCR cloning | |
| - | + | <li>PCR site-directed mutagenesis | |
| + | <li>Extraction of genome DNA for PCR cloning | ||
| + | </ul> | ||
| + | </div> | ||
| + | </html> | ||
Revision as of 11:06, 1 October 2010
Protocols
- Preparation of competent cells for heat-shock transformation
- Transformation by heat-shock
- Plasmid DNA miniprep
- 3A BioBrick assembly
- PCR cloning
- PCR site-directed mutagenesis
- Extraction of genome DNA for PCR cloning
