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Team:Osaka/Protocols
From 2010.igem.org
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==Protocols== | ==Protocols== | ||
| - | + | *Preparation of competent cells for heat-shock transformation | |
| - | + | *Transformation by heat-shock | |
| - | + | *Plasmid DNA miniprep | |
| - | + | * 3A BioBrick assembly | |
| - | + | *PCR cloning | |
| - | + | *PCR site-directed mutagenesis | |
| - | + | *Extraction of genome DNA for PCR cloning | |
Revision as of 12:33, 25 September 2010
| Home | Team | Project | Submitted Parts | Modeling | Protocols | Notebook | Safety |
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Protocols
- Preparation of competent cells for heat-shock transformation
- Transformation by heat-shock
- Plasmid DNA miniprep
- 3A BioBrick assembly
- PCR cloning
- PCR site-directed mutagenesis
- Extraction of genome DNA for PCR cloning
