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Team:Osaka/Project pga
From 2010.igem.org
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<p> | <p> | ||
Poly-gamma-glutamic acid (γ-PGA) is an interesting biopolymer. | Poly-gamma-glutamic acid (γ-PGA) is an interesting biopolymer. | ||
| - | It can be found in "natto", a Japanese traditional food made of soybeans. Bacillus subtilis ferments soybeans and | + | It can be found in "natto", a Japanese traditional food made of soybeans. Bacillus subtilis ferments soybeans and creates sticky-ness product, PGA, to protect itself from other microorganisms. |
| - | It is a promising polymer, because it has many | + | It is a promising polymer, because it has many functions.(For instance, highly water-holding, edible, biodegradable...)</p> |
<img src="https://static.igem.org/mediawiki/2010/c/cd/Natto_01.png" height="180" width="195" alt="Fig.1"><img src="https://static.igem.org/mediawiki/2010/a/a5/Natto_02.png" height="180" width="151" alt="Fig.2"><img src="https://static.igem.org/mediawiki/2010/c/c0/Natto_03.png" height="180" width="223" alt="Fig.3"> | <img src="https://static.igem.org/mediawiki/2010/c/cd/Natto_01.png" height="180" width="195" alt="Fig.1"><img src="https://static.igem.org/mediawiki/2010/a/a5/Natto_02.png" height="180" width="151" alt="Fig.2"><img src="https://static.igem.org/mediawiki/2010/c/c0/Natto_03.png" height="180" width="223" alt="Fig.3"> | ||
<p> | <p> | ||
| - | Poly glutamate synthetase(PGS) is necessary to | + | Poly glutamate synthetase(PGS) is necessary to produce PGA in Escherichia coli. PGS gene includes three ORF (open reading flame),which are pgsB, pgsC, and pgsA. |
These ORFs code three essential enzymes to create PGA. | These ORFs code three essential enzymes to create PGA. | ||
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| - | To increase the amount of PGA production, co-expression of glutamate racemase and PGS is | + | To increase the amount of PGA production, co-expression of glutamate racemase and PGS is needed. |
</p> | </p> | ||
<p> | <p> | ||
Revision as of 08:53, 1 October 2010
Poly-gamma-glutamic acid
Poly-gamma-glutamic acid (γ-PGA) is an interesting biopolymer. It can be found in "natto", a Japanese traditional food made of soybeans. Bacillus subtilis ferments soybeans and creates sticky-ness product, PGA, to protect itself from other microorganisms. It is a promising polymer, because it has many functions.(For instance, highly water-holding, edible, biodegradable...)
Poly glutamate synthetase(PGS) is necessary to produce PGA in Escherichia coli. PGS gene includes three ORF (open reading flame),which are pgsB, pgsC, and pgsA. These ORFs code three essential enzymes to create PGA.
PGA contains both D and L glutamate.
To increase the amount of PGA production, co-expression of glutamate racemase and PGS is needed.
PGS is originally localized in cell membrane, and proposed to produce PGA from D,L-Glutamic acid. Bacillus subtilis has enzymes to biodegrade PGA; however, E. coli does not. Therefore, we want E. coli to produce PGA for greening of the desert.
