Lac Operon Control
Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG
Chitin
Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886
Chitin synthesis Pathway (image): [1]
Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long
Uses for Chitin: http://www.gmp-chitosan.com/en/products-services/chitin.html
Chitin Synthesis
Chitin Synthase Gene (CHS3): http://www.ncbi.nlm.nih.gov/gene/852311
CHS3 polymerizes: http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465
Chitin Staining (Calcofluor)
Effect on e. coli: http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf
Staining of Biofilm Polysaccharides- http://tinyurl.com/36pn4za
Inhibition of Chitin Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/
Data sheet: http://tinyurl.com/283wax2
Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html
Apoptosis
partsregistry: http://partsregistry.org/Cell_death
Live/Dead Staining aka Viability/Cytotoxicity Assay
DMAO(Live), EtD-III(Dead)
Keio Collection - KnockOuts
Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html
Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp
Method: http://www.pnas.org/content/97/12/6640.full.pdf+html
Journal Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html
Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls
ChiA (Chitinase) Knockout
Summary of e. coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
Chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
ydgg/tqsA (AI2-Transporter) Knockout
Knockout biofilm formation: http://jb.asm.org/cgi/content/short/188/2/587, http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593
more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf
BIF (Biological Imaging Facility)
List of Equipment: http://www.northwestern.edu/bioimaging/equip.html
Methods: http://www.northwestern.edu/bioimaging/methods.html
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