http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Protocol/Methanol_Fixation&feed=atom&action=historyTeam:Northwestern/Protocol/Methanol Fixation - Revision history2024-03-28T10:07:11ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Protocol/Methanol_Fixation&diff=122465&oldid=prevTimsterxox: New page: # Rinse slide with ethanol and flame # Rinse slide with PBS and dry with KimWipe # Apply cell sample to microscope slide and let air dry # Submerge in -20C absolute methanol for 5-10 min #...2010-10-23T01:25:40Z<p>New page: # Rinse slide with ethanol and flame # Rinse slide with PBS and dry with KimWipe # Apply cell sample to microscope slide and let air dry # Submerge in -20C absolute methanol for 5-10 min #...</p>
<p><b>New page</b></p><div># Rinse slide with ethanol and flame<br />
# Rinse slide with PBS and dry with KimWipe<br />
# Apply cell sample to microscope slide and let air dry<br />
# Submerge in -20C absolute methanol for 5-10 min<br />
# Wash 3 times with 1X TBS (submerge in 1X TBS for 5 minutes per wash)<br />
** '''Do not let cells dry for the rest of the procedure'''<br />
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Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]<br />
[[Category:Protocol]]</div>Timsterxox