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Protocol

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Contents 1 Bacterial Work 1.1 Transformation 1.2 O/N Culture 1.3 Preparation of Competent Cells 1.4 LB Media 1.5 Preparing Plates 2 DNA 2.1 Restriction Enzyme Digests 2.2 Ligations 2.3 Mini Prep

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

DNA

Restriction Enzyme Digests

Ligations

Mini Prep