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Revision as of 17:34, 23 June 2010 (view source) Asamelson (Talk | contribs) ← Older edit		Revision as of 17:34, 23 June 2010 (view source) Asamelson (Talk | contribs) (→Prepping DNA from the Plates) Newer edit →
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	===[[Quikchange (from primers to colonies!)]]===		===[[Quikchange (from primers to colonies!)]]===

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Revision as of 17:34, 23 June 2010

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Protocol

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Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 DNA 3.1 Restriction Enzyme Digests 3.2 Ligations 3.3 Mini Prep

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

DNA

Restriction Enzyme Digests

Ligations

Mini Prep