Team:Northwestern/Protocol

From 2010.igem.org

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(Reagents)
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==Reagents==
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=='''Reagents'''==
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[[Reagents]]
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=='''Cell Staining'''==
=='''Cell Staining'''==
===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===
===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===

Revision as of 16:19, 29 June 2010

Tech Institute

Home Brainstorm Team Acknowledgements Project Side Project Parts Notebook Calendar Protocol Safety Links References Media Contact


Contents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit