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(Prepping DNA from the kit plates)
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== '''DNA''' ==
== '''DNA''' ==
[[Team:Northwestern/protocol/|Prepping DNA from the kit plates]]
===[[Prepping DNA from the kit plates]]===
===[[Prepping DNA from the kit plates]]===

Revision as of 18:34, 16 October 2010

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Prepping DNA from the kit plates

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Ethanol Precipitation

New Part Design(PCR)

Bacterial Work


O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Glycerol Stocks


Restriction Enzyme Digests

Plasmid Construction

3A Assembly


Mini Prep


Confocal Microscopy

Fluorescence Microscopy



Cell Staining

Rhodamine-Conjugated Chitin Probe

Methanol Fixation

  1. Rinse slide with ethanol and flame
  2. Rinse slide with PBS and dry with KimWipe
  3. Apply cell sample to microscope slide and let air dry
  4. Submerge in -20C absolute methanol for 5-10 min
  5. Wash 3 times with 1X TBS (submerge in 1X TBS for 5 minutes per wash)
    • Do not let cells dry for the rest of the procedure

LIVE/DEAD® BacLight - Bacterial Viability Kit