Team:Northwestern/Protocol

From 2010.igem.org

(Difference between revisions)
(Prepping DNA from the Plates)
 
(53 intermediate revisions not shown)
Line 1: Line 1:
-
[[Image:LOGOBANNER1.jpg|930px|center|link=http://wikipedia.org|caption]]
+
__NOTOC__
 +
 
 +
 
 +
<html>
 +
<style>
 +
/* Wiki Hacks - START */
 +
#globalWrapper {
 +
    background-color: transparent;
 +
    padding-bottom:0px;
 +
    border: none;
 +
    }
 +
#top-section {
 +
    height: 0px;
 +
    margin-top: 0px;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    margin-bottom: 0 !important;
 +
    margin-bottom: 0px;
 +
    padding:0;
 +
    border: 1;
 +
    }
 +
#p-logo {
 +
    height:0px;
 +
    overflow:hidden;
 +
    border:none;
 +
    }
 +
#search-controls {
 +
    overflow:hidden;
 +
    display:none;
 +
    background: none;
 +
    position: absolute;
 +
    top: 100px;
 +
    right: 40px;
 +
    }         
 +
 
 +
 
 +
/* Wiki Hacks - END */
 +
 
 +
 
 +
#content {
 +
    background-color: transparent;
 +
    width:99%;
 +
    position: relative;
 +
    float: center;
 +
    border: 0px;
 +
}
 +
 
 +
#menubar {
 +
background-color:transparent;
 +
font-size:85%;
 +
line-height:1em;
 +
position:absolute;
 +
top:10px;
 +
white-space:nowrap;
 +
width:50%;
 +
z-index:5;
 +
height:25px;
 +
overflow:hidden;
 +
}
 +
 
 +
#menubar li a {
 +
color:#000000;
 +
}
 +
 
 +
.left-menu {
 +
left:0;
 +
padding-left:13px;
 +
}
 +
.right-menu  {
 +
right:0;
 +
}
 +
 
 +
.firstHeading { display:none; }
 +
}
 +
 
 +
 
 +
 
 +
 
 +
#footer-box {
 +
background-color: transparent;
 +
color: #000000;
 +
border: 0px;
 +
margin:0 auto;
 +
padding:13px 5px;
 +
width:auto;
 +
margin-top:-1px;
 +
}
 +
 
 +
#footer-box a {
 +
        background-color: transparent;
 +
color: #000000;
 +
font-size:90%;
 +
 
 +
}
 +
/* Wiki Hacks - END */
 +
</style>
 +
</html>
 +
 
 +
<html>
 +
<head>
 +
<style>
 +
body {
 +
  background: #EFCDF8; <!-- EEDCC8 url(http://2010.igem.org/wiki/images/3/38/BG1.jpg) repeat-y; -->
 +
}
 +
</style>
 +
</head>
 +
</html>
 +
 
 +
[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
-
{|- style="background:#F5EFFB; color:#8B008B" cellpadding="1" cellspacing="1" border="1" bordercolor="#F5EFFB" width="97%" align="center"
+
{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
-
!align="center"|[[Team:Northwestern|<span style="color:#4B088A;">Home</span>]]
+
!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
-
!align="center"|[[Team:Northwestern/Team|<span style="color:#4B088A;">Team</span>]]
+
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
-
!align="center"|[[Team:Northwestern/Project|<span style="color:#4B088A;">Project</span>]]
+
!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
-
!align="center"|[[Team:Northwestern/Parts|<span style="color:#4B088A;">Parts</span>]]
+
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
-
!align="center"|[[Team:Northwestern/Notebook|<span style="color:#4B088A;">Notebook</span>]]
+
!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
-
!align="center"|[[Team:Northwestern/Safety|<span style="color:#4B088A;">Safety</span>]]
+
!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
-
!align="center"|[[Team:Northwestern/Calendar|<span style="color:#4B088A;">Calendar</span>]]
+
!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
-
!align="center"|[[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]
+
!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
 +
!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
 +
!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
 +
!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
 +
!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
 +
!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
 +
!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
 +
!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
|}
|}
-
'''Protocol'''
+
{| align="center" border="0" width="75%"
 +
|
 +
=='''Protocols'''==
-
http://ginkgobioworks.com/support/
+
== '''DNA''' ==
-
Formatting: http://meta.wikimedia.org/wiki/Help:Wikitext_examples
+
[[Team:Northwestern/Protocol/PDNA|Prepping DNA from the kit plates]]
-
OpenWetWare Protocols: http://openwetware.org/wiki/Protocols
+
[[Team:Northwestern/Protocol/Quikchange (from primers to colonies!|Quikchange: Primers to Colonies]]
 +
[[Team:Northwestern/Protocol/Kit to Stock Plasmid|Kit to Stock Plasmid]]
-
== DNA ==
+
[[Team:Northwestern/Protocol/Ethanol Precipitation|Ethanol Precipitation]]
 +
[[Team:Northwestern/Protocol/New Part Design(PCR)|New Part Design]]
-
===[[Prepping DNA from the kit plates]]===
+
=='''Bacterial Work'''==
-
===[[Quikchange (from primers to colonies!)]]===
+
[[Team:Northwestern/Protocol/Transformation|Transformation]]
-
==Bacterial Work==
+
[[Team:Northwestern/Protocol/O/N Culture|Overnight Cultures]]
-
===[[Transformation]]===
+
[[Team:Northwestern/Protocol/Preparation of Competent Cells|Preparation of Competent Cells]]
-
===[[O/N Culture]]===
+
[[Team:Northwestern/Protocol/LB Media|LB Media]]
-
===[[Preparation of Competent Cells]]===
+
[[Team:Northwestern/Protocol/Preparing Plates|Preparing Plates]]
-
===[[LB Media]]===
+
[[Team:Northwestern/Protocol/Glycerol Stocks|Glycerol Stocks]]
-
===[[Preparing Plates]]===
+
=='''Assembly'''==
-
==DNA==
+
[[Team:Northwestern/Protocol/Restriction Enzyme Digests|Restriction Enzyme Digests]]
-
===[[Restriction Enzyme Digests]]===
+
[[Team:Northwestern/Protocol/Plasmid Construction|Plasmid Construction]]
-
===[[Ligations]]===
+
[[Team:Northwestern/Protocol/3A Assembly|3A Assembly]]
-
===[[Mini Prep]]===
+
 
 +
[[Team:Northwestern/Protocol/Ligations|Ligations]]
 +
 
 +
[[Team:Northwestern/Protocol/Mini Prep|Qiagen Mini Prep]]
 +
 
 +
=='''Microscopy'''==
 +
 
 +
[[Team:Northwestern/Protocol/Confocal Microscopy|Confocal Microscopy]]
 +
 
 +
[[Team:Northwestern/Protocol/Fluorescence Microscopy|Fluorescence Microscopy]]
 +
 
 +
=='''Reagents'''==
 +
 
 +
[[Team:Northwestern/Protocol/Reagents|Reagents]]
 +
 
 +
=='''Cell Staining'''==
 +
 
 +
[[Team:Northwestern/Protocol/Rhodamine-Conjugated Chitin Probe|Rhodamine-Conjugated Chitin Probe]]
 +
 
 +
[[Team:Northwestern/Protocol/Methanol Fixation|Methanol Fixation]]
 +
 
 +
[[Team:Northwestern/Protocol/LIVE/DEAD® BacLight - Bacterial Viability Kit|Live/Dead Baclight]]
 +
 
 +
|}

Latest revision as of 01:23, 27 October 2010


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Protocols

DNA

Prepping DNA from the kit plates

Quikchange: Primers to Colonies

Kit to Stock Plasmid

Ethanol Precipitation

New Part Design

Bacterial Work

Transformation

Overnight Cultures

Preparation of Competent Cells

LB Media

Preparing Plates

Glycerol Stocks

Assembly

Restriction Enzyme Digests

Plasmid Construction

3A Assembly

Ligations

Qiagen Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Conjugated Chitin Probe

Methanol Fixation

Live/Dead Baclight