Team:Northwestern/Protocol

From 2010.igem.org

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===[[Preparing Plates]]===
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===[[Glycerol Stocks]]==
=='''Assembly'''==
=='''Assembly'''==

Revision as of 16:29, 2 July 2010

Tech Institute

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Contents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

=Glycerol Stocks

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit