Team:Northwestern/Protocol

From 2010.igem.org

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(Reagents)
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=='''Reagents'''==
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==Reagents==
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[[Reagents]]
=='''Cell Staining'''==
=='''Cell Staining'''==
===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===
===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===

Revision as of 16:20, 29 June 2010

Tech Institute

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Contents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit