Team:Northwestern/Protocol

From 2010.igem.org

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[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{|- style="background:#F5EFFB; color:#8B008B" cellpadding="1" cellspacing="1" border="1" bordercolor="#F5EFFB" width="97%" align="center"
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{|- style="background:#FFFFFF; color:#000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="97%" align="center"
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!align="center"|[[Team:Northwestern|<span style="color:#4B088A;">Home</span>]]
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!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
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!align="center"|[[Team:Northwestern/Team|<span style="color:#4B088A;">Team</span>]]
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!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]
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!align="center"|[[Team:Northwestern/Project|<span style="color:#4B088A;">Project</span>]]
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!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]
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!align="center"|[[Team:Northwestern/Parts|<span style="color:#4B088A;">Parts</span>]]
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!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
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!align="center"|[[Team:Northwestern/Notebook|<span style="color:#4B088A;">Notebook</span>]]
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!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
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!align="center"|[[Team:Northwestern/Safety|<span style="color:#4B088A;">Safety</span>]]
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!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]
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!align="center"|[[Team:Northwestern/Calendar|<span style="color:#4B088A;">Calendar</span>]]
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!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
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!align="center"|[[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]
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!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
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Revision as of 00:02, 28 June 2010

Tech Institute

Home Team Project Parts Notebook Safety Calendar Protocol

Protocol

http://ginkgobioworks.com/support/

Formatting: http://meta.wikimedia.org/wiki/Help:Wikitext_examples

OpenWetWare Protocols: http://openwetware.org/wiki/Protocols


Contents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep