Team:Northwestern/Project/Modeling

Modeling

Using simple enzyme kinetics equations, we elected to mathematically simulate the following model:

The picture's great, but define what each of the variables mean!

Matlab was used to generate a theoretical model where IPTG would diffuse down the biofilm as according to Fick's Law of Diffusion.

The extracellular substrate concentration was assumed to be much greater than the uptake/use, and so would diffuse in at a constant rate.

This model was fitted with empirical data using cp-lacpi-gfp to estimate the effect of varying cp, lacpi, and rbs on enzyme and final product production.