Team:Northwestern/Project/Modeling

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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Modeling"><img width="100" class="icon" src="http://2010.igem.org/wiki/images/a/a7/NU_modeling.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Chassis"><img width="100" class="icon" src="http://2010.igem.org/wiki/images/a/a6/Nuchassis.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Lac"><img width="100" class="icon" src="http://2010.igem.org/wiki/images/1/18/Nulac.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Chitin Synthesis"><img width="100" class="icon" src="http://2010.igem.org/wiki/images/4/4e/Nuchitin.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Apoptosis"><img width="100" class="icon" src="http://2010.igem.org/wiki/images/c/cb/Nuapop.jpg"></a></td>
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<td align="center">Modeling</td>
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<td align="center">Chassis</td>
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<td align="center">Induction</td>
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<td align="center">Chitin</td>
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<td align="center">Apoptosis</td>
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Revision as of 15:50, 23 October 2010


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Modeling Chassis Induction Chitin Apoptosis


Using simple enzyme kinetics equations, we elected to mathematically simulate the following model:

SuperModeling.jpg

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Matlab was used to generate a theoretical model where IPTG would diffuse down the biofilm as according to Fick's Law of Diffusion.

The extracellular substrate concentration was assumed to be much greater than the uptake/use, and so would diffuse in at a constant rate.

This model was fitted with empirical data using cp-lacpi-gfp to estimate the effect of varying cp, lacpi, and rbs on enzyme and final product production.