Team:Northwestern/Project/Lac

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
-
[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
+
__NOTOC__
 +
 
 +
 
 +
<html>
 +
<style>
 +
/* Wiki Hacks - START */
 +
#globalWrapper {
 +
    background-color: transparent;
 +
    padding-bottom:0px;
 +
    border: none;
 +
    }
 +
#top-section {
 +
    height: 0px;
 +
    margin-top: 0px;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    margin-bottom: 0 !important;
 +
    margin-bottom: 0px;
 +
    padding:0;
 +
    border: 1;
 +
    }
 +
#p-logo {
 +
    height:0px;
 +
    overflow:hidden;
 +
    border:none;
 +
    }
 +
#search-controls {
 +
    overflow:hidden;
 +
    display:none;
 +
    background: none;
 +
    position: absolute;
 +
    top: 100px;
 +
    right: 40px;
 +
    }         
 +
 
 +
 
 +
/* Wiki Hacks - END */
 +
 
 +
 
 +
#content {
 +
    background-color: transparent;
 +
    width:99%;
 +
    position: relative;
 +
    float: center;
 +
    border: 0px;
 +
}
 +
 
 +
#menubar {
 +
background-color:transparent;
 +
font-size:85%;
 +
line-height:1em;
 +
position:absolute;
 +
top:10px;
 +
white-space:nowrap;
 +
width:50%;
 +
z-index:5;
 +
height:25px;
 +
overflow:hidden;
 +
}
 +
 
 +
#menubar li a {
 +
color:#000000;
 +
}
 +
 
 +
.left-menu {
 +
left:0;
 +
padding-left:13px;
 +
}
 +
.right-menu  {
 +
right:0;
 +
}
 +
 
 +
.firstHeading { display:none; }
 +
}
 +
 
 +
 
 +
 
 +
 
 +
#footer-box {
 +
background-color: transparent;
 +
color: #000000;
 +
border: 0px;
 +
margin:0 auto;
 +
padding:13px 5px;
 +
width:auto;
 +
margin-top:-1px;
 +
}
 +
 
 +
#footer-box a {
 +
        background-color: transparent;
 +
color: #000000;
 +
font-size:90%;
 +
 
 +
}
 +
/* Wiki Hacks - END */
 +
</style>
 +
</html>
 +
 
<html>
<html>
<head>
<head>
<style>
<style>
body {
body {
-
   background: #EFCDF8
+
   background: #EFCDF8; <!-- EEDCC8 url(http://2010.igem.org/wiki/images/3/38/BG1.jpg) repeat-y; -->
}
}
</style>
</style>
</head>
</head>
</html>
</html>
 +
 +
[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
 +
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
-
{|- style="background:#FFFFFF; style="font-size: 87%;" color:#000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="97%" align="center"
+
{|- style="font-size: 87%; background: #AFC7C7; color: #000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
-
!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
+
!align="center"|[[Team:Northwestern|<font color="#FFFFFF">'''Home'''</font>]]
-
!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]
+
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#FFFFFF">'''Brainstorm'''</font>]]
-
!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]
+
!align="center"|[[Team:Northwestern/Team|<font color="#FFFFFF">'''Team'''</font>]]
-
!align="center"|[[Team:Northwestern/Acknowledgements|<span style="color:#000000;">'''Acknowledgements'''</span>]]
+
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#FFFFFF">'''Acknowledgements'''</font>]]
-
!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]
+
!align="center"|[[Team:Northwestern/Project|<font color="#2B3856">'''Project'''</font>]]
-
!align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Side Project'''</span>]]
+
!align="center"|[[Team:Northwestern/SideProject|<font color="#FFFFFF">'''Human Practices'''</font>]]
-
!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
+
!align="center"|[[Team:Northwestern/Parts|<font color="#FFFFFF">'''Parts'''</font>]]
-
!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
+
!align="center"|[[Team:Northwestern/Notebook|<font color="#FFFFFF">'''Notebook'''</font>]]
-
!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
+
!align="center"|[[Team:Northwestern/Calendar|<font color="#FFFFFF">'''Calendar'''</font>]]
-
!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
+
!align="center"|[[Team:Northwestern/Protocol|<font color="#FFFFFF">'''Protocol'''</font>]]
-
!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]
+
!align="center"|[[Team:Northwestern/Safety|<font color="#FFFFFF">'''Safety'''</font>]]
-
!align="center"|[[Team:Northwestern/Links|<span style="color:#000000;">'''Links'''</span>]]
+
!align="center"|[[Team:Northwestern/Links|<font color="#FFFFFF">'''Links'''</font>]]
-
!align="center"|[[Team:Northwestern/References|<span style="color:#000000;">'''References'''</span>]]
+
!align="center"|[[Team:Northwestern/References|<font color="#FFFFFF">'''References'''</font>]]
-
!align="center"|[[Team:Northwestern/Media|<span style="color:#000000;">'''Media'''</span>]]
+
!align="center"|[[Team:Northwestern/Media|<font color="#FFFFFF">'''Media'''</font>]]
-
!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]
+
!align="center"|[[Team:Northwestern/Contact|<font color="#FFFFFF">'''Contact'''</font>]]
|}
|}
 +
 +
{| align="center" border="0" width="75%"
 +
|align="left" valign="top" width="80%" style="padding: 5"|
For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information.
For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information.
Line 35: Line 138:
The effectiveness of these constructs were tested in the modeling section.
The effectiveness of these constructs were tested in the modeling section.
 +
 +
|}

Revision as of 04:06, 23 October 2010


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information.

The induction construct consisted of a constitutive promoter (J23100, J23104, or J23105), a combination part of Ribosome Binding Site, Lac Repressor, double Terminators, and Lac Promoter (Q01121, Q04121), and Ribosome Binding Sequence (B0034, B0031, B0032).

Multiple versions of each part were used to generate a wide range of repressor concentrations, so as to allow for variation between the induction time-delay and degree of Apoptosis protein and Chitin Synthase synthesis.

The effectiveness of these constructs were tested in the modeling section.